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Disruption of the NHR4 domain structure in AML1-ETO abrogates SON binding and promotes leukemogenesis
- Source :
- Ahn, E-Y, Yan, M, Malakhova, O A, Lo, M-C, Boyapati, A, Ommen, H B, Hines, R, Hokland, P & Zhang, D-E 2008, ' Disruption of the NHR4 domain structure in AML1-ETO abrogates SON binding and promotes leukemogenesis. ', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 44, pp. 17103-8 . https://doi.org/10.1073/pnas.0802696105
- Publication Year :
- 2008
-
Abstract
- Udgivelsesdato: 2008-Nov-4 AML1-ETO is generated from t(8;21)(q22;q22), which is a common form of chromosomal translocation associated with development of acute myeloid leukemia (AML). Although full-length AML1-ETO alone fails to promote leukemia because of its detrimental effects on cell proliferation, an alternatively spliced isoform, AML1-ETO9a, without its C-terminal NHR3/NHR4 domains, strongly induces leukemia. However, full-length AML1-ETO is a major form of fusion product in many t(8;21) AML patients, suggesting additional molecular mechanisms of t(8;21)-related leukemogenesis. Here, we report that disruption of the zinc-chelating structure in the NHR4 domain of AML1-ETO by replacing only one critical amino acid leads to rapid onset of leukemia, demonstrating that the NHR4 domain with the intact structure generates inhibitory effects on leukemogenesis. Furthermore, we identified SON, a DNA/RNA-binding domain containing protein, as a novel NHR4-interacting protein. Knock-down of SON by siRNA resulted in significant growth arrest, and disruption of the interaction between AML1-ETO and endogenous SON rescued cells from AML1-ETO-induced growth arrest, suggesting that SON is an indispensable factor for cell growth, and AML1-ETO binding to SON may trigger signals inhibiting leukemogenesis. In t(8;21) AML patient-derived primary leukemic cells and cell lines, abnormal cytoplasmic localization of SON was detected, which may keep cells proliferating in the presence of full-length AML1-ETO. These results uncovered the crucial role of the NHR4 domain in determination of cellular fate during AML1-ETO-associated leukemogenesis.
- Subjects :
- Oncogene Proteins, Fusion
Chromosomes, Human, Pair 21
Chromosomal translocation
Biology
Transfection
Minor Histocompatibility Antigens
Mice
RUNX1 Translocation Partner 1 Protein
hemic and lymphatic diseases
medicine
Animals
Humans
neoplasms
Cell Proliferation
Multidisciplinary
Binding Sites
Cell growth
Myeloid leukemia
U937 Cells
Biological Sciences
medicine.disease
Molecular biology
Cell biology
Protein Structure, Tertiary
DNA-Binding Proteins
Leukemia
Leukemia, Myeloid, Acute
Cell culture
Core Binding Factor Alpha 2 Subunit
K562 Cells
K562 cells
Chromosomes, Human, Pair 8
HeLa Cells
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 105
- Issue :
- 44
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....65f861dbe7ec14746f020386c69e99a6