Results of a screening programme to identify plants or plant extracts that inhibit ruminal protein degradation

One aim of the EC Framework V project, ’Rumen-up’ (QLK5-CT-2001-00 992), was to find plants or plant extracts that would inhibit the nutritionally wasteful degradation of protein in the rumen. A total of 500 samples were screenedin vitrousing14C-labelled casein in a 30-min incubation with ruminal digesta. Eight were selected for further investigation using a batch fermentation system and soya protein and bovine serum albumin as proteolysis substrates; proteolysis was monitored over 12 h by the disappearance of soluble protein and the production of branched SCFA and NH3. Freeze-dried, ground foliage ofPeltiphyllum peltatum,Helianthemum canum,Arbutus unedo,Arctostaphylos uva-ursiandKnautia arvensisinhibited proteolysis (P Daucus carota,Clematis vitalbaandErica arboreahad little effect. Inhibition by the first four samples appeared to be caused by the formation of insoluble tannin–protein complexes. The samples were rich in phenolics and inhibition was reversed by polyethyleneglycol. In contrast,K.arvensiscontained low concentrations of phenolics and no tannins, had no effect in the 30-min assay, yet inhibited the degradation rate of soluble protein (by 14 %,P P 0·05) without precipitating protein in the 12-h batch fermentation. The effects showed some resemblance to those obtained in parallel incubations containing 3 μm-monensin, suggesting thatK.arvensismay be a plant-derived feed additive that can suppress growth and activity of key proteolytic ruminal micro-organisms in a manner similar to that already well known for monensin.