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Simultaneous analysis of select pharmaceuticals and personal care products in fish tissue using pressurized liquid extraction combined with silica gel cleanup
- Source :
- Journal of chromatography. A. 1218(37)
- Publication Year :
- 2011
-
Abstract
- Analytical improvements were developed and validated for measuring select personal care products (PCPs) and two pharmaceuticals in fish tissue. The method was validated using fortified fillet tissue for twelve PCPs including fragrance materials, alkylphenols, photo initiators, and triclosan as well as two pharmaceuticals including carbamazepine (anti-seizure) and diazepam (anti-convulsant). The analytical method utilized pressurized liquid extraction (PLE) combined with silica gel cleanup, gel permeation chromatography, and gas chromatography ion-trap tandem mass spectrometry. Silica gel cleanup was combined with the PLE to produce one automated extraction/cleanup technique. This analytical improvement served to reduce the incurred cost, time, and loss of potential target analytes associated with independent cleanup steps. The combined extraction/cleanup technique resulted in an average increase of 10% in analyte recoveries. Average triplicate recoveries and relative standard deviations for the entire method, using 2.5 g of fish fillet tissue, were 92 ± 9% (recoveries ranged from 64 to 131%). The sensitivity of the analytical methods was improved by optimizing the resonant collision induced dissociation energy to the hundredths place (0.01 V). Improvements in ion production range from 24 to 122% for six of the 12 PCPs. Statistically derived method detection limits (MDLs) were also lowered on average by a factor of 8 and ranged from 1.2 to 38 ng/g wet weight. MDLs for carbamazepine and diazepam were 18 and 3.7 ng/g wet weight, respectively. Galaxolide and tonalide were measured in an environmental sample at concentrations of 81 and 5.5 ng/g wet weight, respectively.
- Subjects :
- Analyte
Silica Gel
Cosmetics
Chemical Fractionation
Biochemistry
Environmental impact of pharmaceuticals and personal care products
Sensitivity and Specificity
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
Gel permeation chromatography
chemistry.chemical_compound
Animals
Galaxolide
Detection limit
Chromatography
Diazepam
Silica gel
Organic Chemistry
Fishes
Fish fillet
Reproducibility of Results
General Medicine
Drug Residues
Carbamazepine
chemistry
Chromatography, Gel
Gas chromatography
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 18733778
- Volume :
- 1218
- Issue :
- 37
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....65c8a5a9f4b21da5d2f7898939ac476e