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Team work and cytopathology molecular diagnosis of solid pancreatic lesions
- Publication Year :
- 2017
-
Abstract
- Pancreatic ductal adenocarcinoma (PDAC) is expected to become the second leading cause of cancer-associated death in the next decade or so. It is widely accepted that tumorigenesis is linked to specific alterations in key genes and pancreatic neoplasms are some of the best characterized at the genomic level. Recent whole-exome and whole-genome sequencing analyses confirmed that PDAC is frequently characterized by mutations in a set of four genes among others: KRAS, TP53, CDKN2A/p16, and SMAD4. Sequencing, for example, is the preferable technique available for detecting KRAS mutations, whereas in situ immunochemistry is the main approach for detecting TP53 gene alteration. Nevertheless, the diagnosis of PDAC is still a clinical challenge, involving adequate acquisition of endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA) and specific pathological assessment from tissue architecture to specific biomolecular tests. The aim of the present review is to provide a complete overview of the current knowledge of the biology of pancreatic cancer as detected by the latest biomolecular techniques and, moreover, to propose a paradigm for strict teamwork collaboration in order to improve the correct use of diagnostic sources.
- Subjects :
- Endoscopic ultrasound
Male
Pathology
Radiology, Nuclear Medicine and Imaging
Key genes
endocrine system diseases
Molecular marker
Bioinformatics
medicine.disease_cause
Endosonography
0302 clinical medicine
CDKN2A
Nuclear Medicine and Imaging
Smad4 Protein
medicine.diagnostic_test
Gastroenterology
molecular diagnostic
Immunohistochemistry
Molecular Diagnostic Techniques
Pancreatic Ductal
030220 oncology & carcinogenesis
030211 gastroenterology & hepatology
Female
KRAS
Radiology
Carcinoma, Pancreatic Ductal
medicine.medical_specialty
Pancreatic ductal adenocarcinoma
molecular markers
Settore MED/12 - GASTROENTEROLOGIA
review
pancreatic ductal adenocarcinoma
Sensitivity and Specificity
03 medical and health sciences
Endoscopic ultrasound-guided fine-needle aspiration
Molecular diagnostic
Molecular markers
Review
Pancreatic cancer
medicine
Cyclin-Dependent Kinase Inhibitor p18
Humans
endoscopic ultrasound-guided fine-needle aspiration
Neoplasm Invasiveness
Endoscopic Ultrasound-Guided Fine Needle Aspiration
Cyclin-Dependent Kinase Inhibitor p16
Neoplasm Staging
Patient Care Team
business.industry
Pancreatic Neoplasms
Carcinoma
medicine.disease
digestive system diseases
Cytopathology
Carcinogenesis
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....65b9c4e3835875ed0c82f20801a18fa2