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High magnesium inhibits human osteoblast differentiation in vitro
- Source :
- Magnesium research. 24(1)
- Publication Year :
- 2011
-
Abstract
- Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1 mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1 mM) and high (5.0 mM) concentrations of magnesium. We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved. We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations – among which calcium – by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.
- Subjects :
- medicine.medical_specialty
Clinical Biochemistry
chemistry.chemical_element
Calcium
Nitric Oxide
Biochemistry
Bone remodeling
Osteogenesis
Internal medicine
Cell Line, Tumor
Bone cell
medicine
Extracellular
Humans
Magnesium
Molecular Biology
Osteoblasts
Chemistry
Osteoblast
Cell Differentiation
Alkaline Phosphatase
medicine.anatomical_structure
Endocrinology
Alkaline phosphatase
Homeostasis
Subjects
Details
- ISSN :
- 19524021
- Volume :
- 24
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Magnesium research
- Accession number :
- edsair.doi.dedup.....65b7e15e48146c315d9a3c2523ced0a8