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CRISPR-induced exon skipping is dependent on premature termination codon mutations
- Source :
- Genome Biology, Genome Biology, Vol 19, Iss 1, Pp 1-5 (2018)
- Publication Year :
- 2018
-
Abstract
- In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing. Electronic supplementary material The online version of this article (10.1186/s13059-018-1532-z) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
lcsh:QH426-470
Short Report
Biology
medicine.disease_cause
03 medical and health sciences
Exon
0302 clinical medicine
medicine
Missense mutation
CRISPR
Animals
Clustered Regularly Interspaced Short Palindromic Repeats
lcsh:QH301-705.5
CRISPR/Cas9
Genetics
Mutation
Cas9
Exons
Exon skipping
Human genetics
lcsh:Genetics
030104 developmental biology
PTC
lcsh:Biology (General)
Codon, Nonsense
030220 oncology & carcinogenesis
RNA splicing
Rabbits
Subjects
Details
- ISSN :
- 1474760X
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Genome biology
- Accession number :
- edsair.doi.dedup.....65a8017e8142b31ce62c32bb907859ba