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Potential Indexing of the Invasiveness of Breast Cancer Cells by Mitochondrial Redox Ratios
- Source :
- Advances in Experimental Medicine and Biology ISBN: 9783319388083
- Publication Year :
- 2016
- Publisher :
- Springer International Publishing, 2016.
-
Abstract
- The invasive/metastatic potential of cancer cells is an important factor in tumor progression. The redox ratios obtained from ratios of the endogenous fluorescent signals of NADH and FAD, can effectively respond to the alteration of cancer cells in its mitochondrial energy metabolism. It has been shown previously that the redox ratios may predict the metastatic potential of cancer mouse xenografts. In this report, we aimed to investigate the metabolic state represented by the redox ratios of cancer cells in vitro. Fluorescence microscopic imaging technology was used to observe the changes of the endogenous fluorescence signals of NADH and FAD in the energy metabolism pathways. We measured the redox ratios (FAD/NADH) of breast cancer cell lines MDA-MB-231, MDA-MB-468, MCF-7, and SKBR3. We found that the more invasive cancer cells have higher FAD/NADH ratios, largely consistent with previous studies on breast cancer xenografts. Furthermore, by comparing the fluorescence signals of the breast cancer cells under different nutritional environments, including starvation and addition of glutamine, pyruvate and lactate, we found that the redox ratios still effectively distinguished the highly invasive MDA-MB-231 cells from less invasive MCF-7 cells. These preliminary data suggest that the redox ratio may potentially provide a new index to stratefy breast cancer with different degrees of aggressiveness, which could have significance for the diagnosis and treatment of breast cancer.
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
Breast Neoplasms
Mitochondrion
Biology
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Breast cancer
Cell Movement
Rotenone
Internal medicine
Biomarkers, Tumor
Tumor Microenvironment
medicine
Humans
Neoplasm Invasiveness
skin and connective tissue diseases
Flavin adenine dinucleotide
Tumor microenvironment
Uncoupling Agents
Cancer
NAD
medicine.disease
Mitochondria
030104 developmental biology
Microscopy, Fluorescence
chemistry
Tumor progression
SKBR3
030220 oncology & carcinogenesis
Cancer cell
Flavin-Adenine Dinucleotide
MCF-7 Cells
Cancer research
Female
Energy Metabolism
Oxidation-Reduction
Subjects
Details
- ISBN :
- 978-3-319-38808-3
- ISBNs :
- 9783319388083
- Database :
- OpenAIRE
- Journal :
- Advances in Experimental Medicine and Biology ISBN: 9783319388083
- Accession number :
- edsair.doi.dedup.....657fc8401aebff90ac3130b0f2cbf75d