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Cation content in poliovirus-infected HeLa cells
- Source :
- Scopus-Elsevier, Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 1987
- Publisher :
- Society for General Microbiology, 1987.
-
Abstract
- Poliovirus-infected HeLa cells increased their permeability to monovalent ions from the third hour post-infection. At that time infected cells lost their content of potassium ions as measured by efflux of the potassium analogue 86Rb+. The rate of release of 86Rb+ from cells increased as infection proceeded, and was not sensitive to ouabain or quinidine, inhibitors of the Na+/K+ ATPase and Ca2+-induced K+ release system, respectively. The leakage of 86Rb+ was only slightly sensitive to furosemide, an inhibitor of the Na+/K+/Cl− cotransport system, suggesting that the mechanism of release involved an increased passive permeability of the cell membrane. The calcium content or its efflux did not vary significantly in the infected cells. Neither were there alterations in the intracellular pH throughout infection.<br />Ms M. A. Ramos is acknowledged for excellent technical assistance. A.L.R. and J.L.C. are fellows of the Ministerio de Education y Ciencia. CAICYT and FIS are acknowledged for financial support.
- Subjects :
- Quinidine
Intracellular Fluid
Cell Membrane Permeability
Intracellular pH
Sodium-Potassium-Exchanging ATPase
Potassium
chemistry.chemical_element
Biology
Calcium
Ouabain
Furosemide
Virology
Cations
medicine
Humans
Cation content
Na+/K+-ATPase
Symporters
Biological Transport
Hydrogen-Ion Concentration
Rubidium
Molecular biology
Sodium Chloride Symporters
Poliovirus
Biochemistry
chemistry
Plasma membrane Ca2+ ATPase
Carrier Proteins
medicine.drug
HeLa Cells
Plasma membrane
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....6575819df3e4342906f30fa820be3ef0