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Aspidin PB, a novel natural anti-fibrotic compound, inhibited fibrogenesis in TGF-β1-stimulated keloid fibroblasts via PI-3K/Akt and Smad signaling pathways
- Source :
- Chemico-Biological Interactions. 238:66-73
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Keloid is an overgrowth of scar tissue that develops around a wound. The mechanisms of keloid formation and development still remain unknown, and no effective treatment is available. Searching for active natural resources may develop better prevention and treatment approaches for keloids. Aspidin PB is a natural resource with lower toxicity. We explored its effect on the regulation of TGF-β1-induced expression of type I collagen, CTGF, and α-SMA in keloid fibroblasts (KFs). Western blotting was used to detect the expression levels of type I collagen, CTGF, α-SMA, PI-3K/Akt and Smad-dependent and Smad-independent signaling pathway. The effect of aspidin PB on cell viability in human keloid fibroblasts was measured by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide). The percentage of the apoptotic cells was studied by flow cytometry. Based on our results, we revealed that aspidin PB inhibited the production of type I collagen, CTGF, and α-SMA in TGF-β1-induced KFs by blocking PI-3K/Akt signaling pathway. The TGF-β1-mediated phosphorylated levels of Smad2/3 were inhibited by aspidin PB pretreatment. Conclusively, our study suggests that aspidin PB has an inhibitory effect on fibrogenesis in TGF-β1-induced KFs. Our findings imply that aspidin PB has a therapeutic potential to intervene and prevent keloids and other fibrotic diseases.
- Subjects :
- Dryopteris
Pathology
medicine.medical_specialty
Cell Survival
Smad2 Protein
SMAD
Phloroglucinol
Toxicology
Collagen Type I
Transforming Growth Factor beta1
Phosphatidylinositol 3-Kinases
Keloid
medicine
Humans
Viability assay
Phosphorylation
Protein Kinase Inhibitors
Protein kinase B
Cells, Cultured
Phosphoinositide-3 Kinase Inhibitors
Cyclohexanones
Akt/PKB signaling pathway
Chemistry
Connective Tissue Growth Factor
General Medicine
Fibroblasts
medicine.disease
Actins
Plant Leaves
CTGF
Gene Expression Regulation
Cancer research
Proto-Oncogene Proteins c-akt
Type I collagen
Signal Transduction
Transforming growth factor
Subjects
Details
- ISSN :
- 00092797
- Volume :
- 238
- Database :
- OpenAIRE
- Journal :
- Chemico-Biological Interactions
- Accession number :
- edsair.doi.dedup.....65713474a492bd97da63e396ec16c417