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Ganoderic acid A alleviates myocardial ischemia-reperfusion injury in rats by regulating JAK2/STAT3/NF-κB pathway

Authors :
Kejian Shi
Tingting Lin
Fangfang Xia
Yujian Zhang
Yaoyao Cai
Le Liu
Fuli Liu
Yingchao Ye
Source :
International Immunopharmacology. 84:106543
Publication Year :
2020
Publisher :
Elsevier BV, 2020.

Abstract

This study aimed to investigate the protective effect of GanodericacidA (GA) on myocardial ischemia-reperfusion (MIR) injury. The myocardial injury model in rats was established by ligating left anterior descending coronary artery. We measured cardiac hemodynamic, antioxidant enzyme activity, and various biochemical indexes of myocardial tissue, and evaluated myocardial infarction and damage. Further, the expression of JAK2/STAT3/NF-κB signaling pathway-related proteins in myocardial tissue was measured by western blot. The results showed that the myocardial infarction extention was obviously reduced upon GA treatment. Compared with the control group, ischemia-reperfusion rats showed significant increase in lactate dehydrogenase (LDH) and creatine Kinase (CK), which were significantly decreased in GA group. Besides, GA pretreatment effectively decreased the levels of inflammatory cytokines in serum. The phosphorylation of Janus Kinase 2 (JAK2), signal transducer and activator of transcription (STAT3)and Nuclear factor-κB (NF-κB) in reperfusion group were significantly higher than that in control group, which were reversed upon GA treatment. In conclusion, GA may reduce myocardial injury by regulating JAK2/STAT3/NF-κB pathway.

Details

ISSN :
15675769
Volume :
84
Database :
OpenAIRE
Journal :
International Immunopharmacology
Accession number :
edsair.doi.dedup.....6563d1cc33c0cd828010d980da1bac46
Full Text :
https://doi.org/10.1016/j.intimp.2020.106543