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Ganoderic acid A alleviates myocardial ischemia-reperfusion injury in rats by regulating JAK2/STAT3/NF-κB pathway
- Source :
- International Immunopharmacology. 84:106543
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- This study aimed to investigate the protective effect of GanodericacidA (GA) on myocardial ischemia-reperfusion (MIR) injury. The myocardial injury model in rats was established by ligating left anterior descending coronary artery. We measured cardiac hemodynamic, antioxidant enzyme activity, and various biochemical indexes of myocardial tissue, and evaluated myocardial infarction and damage. Further, the expression of JAK2/STAT3/NF-κB signaling pathway-related proteins in myocardial tissue was measured by western blot. The results showed that the myocardial infarction extention was obviously reduced upon GA treatment. Compared with the control group, ischemia-reperfusion rats showed significant increase in lactate dehydrogenase (LDH) and creatine Kinase (CK), which were significantly decreased in GA group. Besides, GA pretreatment effectively decreased the levels of inflammatory cytokines in serum. The phosphorylation of Janus Kinase 2 (JAK2), signal transducer and activator of transcription (STAT3)and Nuclear factor-κB (NF-κB) in reperfusion group were significantly higher than that in control group, which were reversed upon GA treatment. In conclusion, GA may reduce myocardial injury by regulating JAK2/STAT3/NF-κB pathway.
- Subjects :
- Male
STAT3 Transcription Factor
0301 basic medicine
Immunology
Myocardial Reperfusion Injury
Pharmacology
Ventricular Function, Left
Proinflammatory cytokine
Rats, Sprague-Dawley
Lanosterol
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Lactate dehydrogenase
medicine
Animals
Immunology and Allergy
Myocardial infarction
STAT3
Janus kinase 2
biology
business.industry
Myocardium
NF-kappa B
Janus Kinase 2
medicine.disease
030104 developmental biology
chemistry
Heptanoic Acids
030220 oncology & carcinogenesis
STAT protein
biology.protein
Cytokines
Creatine kinase
business
Reperfusion injury
Signal Transduction
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....6563d1cc33c0cd828010d980da1bac46
- Full Text :
- https://doi.org/10.1016/j.intimp.2020.106543