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Induction of apoptosis in prostatic tumor cell line DU145 by staurosporine, a potent inhibitor of protein kinases
- Source :
- The Prostate. 29:69-76
- Publication Year :
- 1996
- Publisher :
- Wiley, 1996.
-
Abstract
- We are interested in studying the possibility of modulating prostatic cell growth by manipulating apoptosis. Here we show that 1 microM staurosporine (STS) induces a human androgen-independent prostatic tumor cell line, DU145, to undergo dramatic changes in morphology and results in programmed cell death. Several genes involved in apoptosis were analyzed for expression in STS-treated and untreated DU145 cells. It was observed that these genes were differentially regulated. The expression level of bcl-2, bcl-xL, Ich-1L remains unchanged in treated and untreated cells. On the other hand, DAD1 and interleukin-1 beta-converting enzyme (ICE) were downregulated while bcl-xs and Ich-1s were upregulated. By blocking bcl-2 gene expression using antisense oligonucleotides, it was determined that the anti-bcl-2 oligonucleotides have no effect on the proliferation of DU145 or STS-treated DU145 cells. These results demonstrate that programmed cell death can be induced in an androgen-independent prostatic cancer cell line and BCL-2 was found not to play an important role in preventing STS-induced apoptosis in the DU145 cell line.
- Subjects :
- Male
Programmed cell death
Time Factors
Cell Survival
Urology
Molecular Sequence Data
Oligonucleotides
bcl-X Protein
Down-Regulation
Apoptosis
Biology
urologic and male genital diseases
Polymerase Chain Reaction
Alkaloids
DU145
Proto-Oncogene Proteins
Tumor Cells, Cultured
medicine
Humans
Staurosporine
Enzyme Inhibitors
Caenorhabditis elegans Proteins
Protein kinase A
Protein Kinase Inhibitors
bcl-2-Associated X Protein
Base Sequence
Kinase
Cell growth
Carcinoma
Caspase 2
Prostatic Neoplasms
Proteins
DNA, Neoplasm
Up-Regulation
Gene Expression Regulation, Neoplastic
Repressor Proteins
Proto-Oncogene Proteins c-bcl-2
Oncology
Cell culture
Caspases
Cancer research
Apoptosis Regulatory Proteins
Cell Division
medicine.drug
Subjects
Details
- ISSN :
- 10970045 and 02704137
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- The Prostate
- Accession number :
- edsair.doi.dedup.....655a3779145b7c39ca1aef59b97cb100