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Dynamic functional characterization and phylogenetic changes due to Long Chain Fatty Acids pulses in biogas reactors
- Source :
- Kougias, P, Treu, L, Campanaro, S, Zhu, X & Angelidaki, I 2016, ' Dynamic functional characterization and phylogenetic changes due to Long Chain Fatty Acids pulses in biogas reactors ', Scientific Reports, vol. 6, 28810 . https://doi.org/10.1038/srep28810, Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- The process stability of biogas plants is often deteriorated by the accumulation of Long Chain Fatty Acids (LCFA). The microbial community shifts due to LCFA disturbances have been poorly understood as the molecular techniques used were not able to identify the genome characteristics of uncultured microorganisms and additionally, the presence of limited number of reference genomes in public databases prevented the comprehension of specific functional roles characterizing these microorganisms. The present study is the first research which deciphers by means of high throughput shotgun sequencing the dynamics of the microbial community during an inhibitory shock load induced by single pulses of unsaturated LCFA at two different concentrations (i.e. 2 g/L-reactor and 3 g/L-reactor). The metagenomic analysis showed that only the microbes associated with LCFA degradation could encode proteins related to “chemotaxis” and “flagellar assembly”, which promoted the ability to move towards the LCFA sources so as to degrade them. Moreover, the syntrophic interactions found between Syntrophomonas sp. together with Methanosarcina sp. were possibly assigned to the menaquinone-electron transfer. Finally, it was proven that a previously exposed to LCFA inoculum is more efficient in the degradation process of LCFA due to the specialization of the microbial consortium.
- Subjects :
- 0301 basic medicine
food.ingredient
archaea
Microorganism
Microbial Consortia
microbiome
shock
010501 environmental sciences
Biology
01 natural sciences
Genome
Article
high throughput sequencing
Microbiology
lipids
03 medical and health sciences
Bioreactors
food
Anaerobic digestion
biogas
Animals
Anaerobic digestion, biogas, microbiome, metagenomics, high throughput sequencing, bacteria, archaea, lipids, shock
bacteria
Phylogeny
0105 earth and related environmental sciences
metagenomics
Multidisciplinary
Shotgun sequencing
Fatty Acids
Microbial consortium
Manure
Syntrophomonas
030104 developmental biology
Microbial population biology
Biochemistry
Metagenomics
Biofuels
Metagenome
Degradation (geology)
Cattle
lipids (amino acids, peptides, and proteins)
Genome, Bacterial
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....6549fcb1cbb95b5eba1f3a09310c42e1