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The modulation of attachment, growth and morphology of cancerous prostate cells by polyelectrolyte nanofilms
- Source :
- Biomaterials, Biomaterials, 2013, 34 (38), pp.10099-108. ⟨10.1016/j.biomaterials.2013.08.093⟩, Biomaterials, Elsevier, 2013, 34 (38), pp.10099-108. ⟨10.1016/j.biomaterials.2013.08.093⟩
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- free download pdf : http://ac.els-cdn.com/S0142961213010715/1-s2.0-S0142961213010715-main.pdf?_tid=af9b5ff8-4bae-11e3-b1b0-00000aab0f27&acdnat=1384270119_77de34b330a3942315169996314d8ce3; International audience; The behaviour of cancerous epithelial prostatic cells (PC3) growing on polyelectrolytes (PE) coatings was compared to the behaviour of immortalized normal prostatic cells (PNT-2). The cell behaviour was evaluated and quantified in terms of initial cell attachment, growth, metabolic activity, morphometry, adhesion, apoptosis and stress related gene expression. Both the anionic PSS (poly(sodium 4-styrenesulphonate))-terminated surface and cationic PAH (poly(allylamine hydrochloride))-terminated surfaces were not cytotoxic. The initial attachment of cells was better on the PAH-terminated surface compared to fibronectin. However, the proliferation rate of PC3 cells was reduced on the PAH-terminated surface and slightly increased on the PSS coatings. Only PAH prevented the clustering phenotype of PC3 and reduced the number of focal adhesion points as compared to fibronectin or PSS coatings. In contrast, none of the PE surfaces significantly affected the biological responses of PNT-2 cells. PAH-terminating films provide a tool to preferentially modulate the growth of some cancerous phenotypes, in this case as a micro-environment that reduces the growth of metastatic PC3 cells.
- Subjects :
- Male
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Materials science
Polymers
Carcinogenesis
Biophysics
Bioengineering
Epithelial cells
02 engineering and technology
010402 general chemistry
01 natural sciences
Cell Line
Allylamine
Focal adhesion
Biomaterials
chemistry.chemical_compound
Animals
Cytotoxic T cell
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
Cell adhesion
Cell proliferation
biology
Cell growth
Prostate
Prostatic Neoplasms
Adhesion
Models, Theoretical
Layer-by-layer
021001 nanoscience & nanotechnology
Polyelectrolytes
Rats
0104 chemical sciences
Fibronectin
chemistry
Cell culture
Mechanics of Materials
Immunology
biology.protein
Ceramics and Composites
0210 nano-technology
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 34
- Issue :
- 38
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....65495fa85c7d05454352bbe88aed8971
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2013.08.093