Additional file 1 of Degradation of epigallocatechin and epicatechin gallates by a novel tannase TanHcw from Herbaspirillum camelliae

Additional file 1: Fig. S1. Alignment of amino acid sequences of microbial tannases and 4 putative tannases in H. camelliae WT00C. * pfam07519: a conserved domain of tannases predicted by NCBI database (https://www.ncbi.nim,nih.gov/conserved domain/tannase); Acinetobacter: A. bayli (Q8RLZ8); Xanthomonas: X. campestris (Q8P8Y5); Agrobacterium: A. fabrurn (Q8UK62); Bradyrhtzobium: B. japonicum (Q89C36); Pseudomonas: P. syringae (Q88IB4); Aspergillus: A. niger (EHA25030); Tan1-4: putative enzymes of Herbaspirillum camelliae. Fig. S2. Kinetic parameter determination of TanHcw at the optimal temperatures and pH. (a) Lineweaver-Burk plot for substrates EGCG at pH7.0 and 40 ºC; (b) Lineweaver-Burk plot for substrates ECG at pH7.0 and 40 ºC; (c) Lineweaver-Burk plot for substrates MG at pH6.0 and 30 ºC. Table S1. Kinetic parameters of TanHcw compared with other tannases.