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Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
- Source :
- Nanomaterials, Volume 11, Issue 5, Nanomaterials, Vol 11, Iss 1266, p 1266 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Recently, the oxidative behavior of methotrexate (MTX) anticancer drug is highly demanded, due to its side effects on healthy cells, despite being a very challenging task. In this study, we have prepared porous NiO material using sodium sulfate as an electronic disorder reagent by hydrothermal method and found it highly sensitive and selective for the oxidation of MTX. The synthesized NiO nanostructures were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. These physical characterizations delineated the porous morphology and cubic crystalline phase of NiO. Different electrochemical approaches have been utilized to determine the MTX concentrations in 0.04 M Britton–Robinson buffer (BRB) at pH 2 using glassy carbon electrode (GCE)-modified with electronically disordered NiO nanostructures. The linear range for MTX using cyclic voltammetry (CV) was found to be from 5 to 30 nM, and the limit of detection (LOD) and limit of quantification (LOQ) were 1.46 nM and 4.86 nM, respectively, whereas the linear range obtained via linear sweep voltammetry (LSV) was estimated as 15–90 nM with LOD and LOQ of 0.819 nM and 2.713 nM, respectively. Additionally, amperometric studies revealed a linear range from 10 to70 nM with LOD and LOQ of 0.1 nM and 1.3 nM, respectively. Importantly, MTX was successfully monitored in pharmaceutical products using the standard recovery method. Thus, the proposed approach for the synthesis of active metal oxide materials could be sued for the determination of other anticancer drugs in real samples and other biomedical applications.
- Subjects :
- Detection limit
Materials science
Scanning electron microscope
General Chemical Engineering
Non-blocking I/O
NiO nanostructures
02 engineering and technology
electrochemical determination
010402 general chemistry
021001 nanoscience & nanotechnology
Electrochemistry
01 natural sciences
Article
methotrexate
Amperometry
0104 chemical sciences
Chemistry
Linear range
Linear sweep voltammetry
General Materials Science
Cyclic voltammetry
sodium sulfate
0210 nano-technology
QD1-999
Nuclear chemistry
Subjects
Details
- ISSN :
- 20794991
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nanomaterials
- Accession number :
- edsair.doi.dedup.....653faf5d1cb996f5cf3ff760b85ca4e9