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Chenopodium Album Prevents Progression of Cell Growth and Enhances Cell Toxicity in Human Breast Cancer Cell Lines
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2, Iss 3, Pp 160-165 (2009), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2009
- Publisher :
- Hindawi Limited, 2009.
-
Abstract
- The present study is aimed to investigate the effects ofChenopodium album(leaves) on the growth of estrogen dependent (MCF-7) and estrogen independent (MDA-MB-468) human breast cancer cell lines. The different solvent extracts (petroleum ether, ethyl acetate and methanol) were assessed for their cytotoxicity using TBE (Trypan blue exclusion) and MTT [3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium] bioassay. These cells were cultured in MEM (minimum essential medium) medium and incubated with the dilution series of extracts (10–100 mg/ml) in CO2incubator at 37°C for 24 h. Among the various extracts studied for two cell lines, methanolic extract ofC. album(leaves) exhibited maximum antibreast cancer activity having IC50(the concentration of an individual compound leading to 50% inhibition) value 27.31 mg/ml against MCF-7 cell line. Significant percent inhibition (94.06%) in the MeOH extract ofC. album(leaves) at 48 h of exposure and concentration 100 mg/ml (p < 0.05) against MCF-7 breast cancer cell line, indicates the presence of some structural moiety responsible for this observed antiproliferative effect. In vivo study and structural elucidation of its bioactive principle are in progress. Our findings highlight the potential of this plant for its possible clinical use to counteract malignancy development as antibreast cancer bioagent.
- Subjects :
- antiproliferative activity
Aging
Stereochemistry
anti breast cancer bioagent
Breast Neoplasms
Biology
Biochemistry
Chenopodium album
chemistry.chemical_compound
In vivo
Cell Line, Tumor
medicine
Humans
Bioassay
Petroleum ether
lcsh:QH573-671
Cytotoxicity
Cell Proliferation
Plant Extracts
Cell growth
lcsh:Cytology
MTT bioassay
TBE bioassay
Cancer
Cell Biology
General Medicine
medicine.disease
Research Papers
Antineoplastic Agents, Phytogenic
Molecular biology
Plant Leaves
chemistry
Cell culture
Female
Trypan blue
Subjects
Details
- Language :
- English
- ISSN :
- 19420994 and 19420900
- Volume :
- 2
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....6536f88307e35ee728f1d7bd1112753a