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Specific simple sugars promote chemotaxis and chemokinesis of corneal endothelial cells
- Source :
- Journal of Cellular Physiology. 157:359-366
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- Bovine corneal endothelial cells showed a strong migratory response to specific simple sugars (D-glucose and sucrose, but not L-glucose, sorbitol, lactose, or D-galactose) at concentrations above 10 mM. Checkerboard analysis of the migratory responses in modified Boyden chambers indicated both chemotactic and chemokinetic effects. Serum starvation of the cultures increased the chemotaxis towards D-glucose and 2-deoxy-D-glucose, but not towards sucrose. Migration to sucrose and glucose was inhibited by chelation of extracellular calcium or by inhibition of Na+,K+ ATPase with ouabain. To date, this migratory response has been found only in corneal endothelial cells. Neither human melanoma cells, human breast carcinoma cells, bovine aortic endothelial cells, nor bovine microvascular endothelial cells migrated towards simple sugars, although all cell types migrated toward fibronectin in chemotaxis assays. After 16–19 passages in culture, bovine corneal endothelial cells retained their ability to migrate towards fibronectin, but lost their ability to migrate towards sugars. This loss of migratory response was accompanied by a sevenfold decrease in Na+,K+ ATPase activity. Although loss of Na+,K+ ATPase activity accompanied the loss of migratory response, pretreatment of cell cultures with 25 mM glucose did not stimulate, but rather lowered Na+,K+ ATPase activity in low or high passage cultures. © 1993 Wiley-Liss, Inc.
- Subjects :
- Sucrose
Physiology
ATPase
medicine.medical_treatment
Clinical Biochemistry
Chemokinesis
Deoxyglucose
Ouabain
Cell Movement
Extracellular
medicine
Animals
Cells, Cultured
biology
Chemotaxis
Growth factor
Endothelium, Corneal
Blood Proteins
Cell Biology
Molecular biology
Fibronectins
Endothelial stem cell
Glucose
Biochemistry
Cell culture
biology.protein
Calcium
Cattle
Sodium-Potassium-Exchanging ATPase
medicine.drug
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....6519b0b5f2fc444f57e83d723f628da1