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FabQ, a Dual-Function Dehydratase/Isomerase, Circumvents the Last Step of the Classical Fatty Acid Synthesis Cycle
- Source :
- Chemistry & Biology. 20(9):1157-1167
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- SummaryIn the classical anaerobic pathway of unsaturated fatty acid biosynthesis, that of Escherichia coli, the double bond is introduced into the growing acyl chain by the FabA dehydratase/isomerase. Another dehydratase, FabZ, functions in the chain elongation cycle. In contrast, Aerococcus viridans has only a single FabA/FabZ homolog we designate FabQ. FabQ can not only replace the function of E. coli FabZ in vivo, but it also catalyzes the isomerization required for unsaturated fatty acid biosynthesis. Most strikingly, FabQ in combination with E. coli FabB imparts the surprising ability to bypass reduction of the trans-2-acyl-ACP intermediates of classical fatty acid synthesis. FabQ allows elongation by progressive isomerization reactions to form the polyunsaturated fatty acid, 3-hydroxy-cis-5, 7-hexadecadienoic acid, both in vitro and in vivo. FabQ therefore provides a potential pathway for bacterial synthesis of polyunsaturated fatty acids.
- Subjects :
- Aerococcus
Stereochemistry
Molecular Sequence Data
Clinical Biochemistry
Isomerase
medicine.disease_cause
Biochemistry
Article
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
Isomerism
Drug Discovery
medicine
Escherichia coli
Fatty Acid Synthase, Type II
Amino Acid Sequence
Isomerases
Peptide sequence
Molecular Biology
Fatty acid synthesis
Hydro-Lyases
030304 developmental biology
chemistry.chemical_classification
Pharmacology
0303 health sciences
biology
030306 microbiology
Computational Biology
General Medicine
Fatty acid synthase
chemistry
Biocatalysis
Dehydratase
Multigene Family
biology.protein
Fatty Acids, Unsaturated
Molecular Medicine
Polyunsaturated fatty acid
Subjects
Details
- ISSN :
- 10745521
- Volume :
- 20
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Chemistry & Biology
- Accession number :
- edsair.doi.dedup.....6512e9b5a9104357b6640d252933c73c
- Full Text :
- https://doi.org/10.1016/j.chembiol.2013.07.007