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Effect of Amelogenin Coating of a Nano-Modified Titanium Surface on Bioactivity
- Source :
- International Journal of Molecular Sciences, Vol 19, Iss 5, p 1274 (2018), International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 19; Issue 5; Pages: 1274
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- The interactions between implants and host tissues depend on several factors. In particular, a growing body of evidence has demonstrated that the surface texture of an implant influences the response of the surrounding cells. The purpose of this study is to develop new implant materials aiming at the regeneration of periodontal tissues as well as hard tissues by coating nano-modified titanium with amelogenin, which is one of the main proteins contained in Emdogain®. We confirmed by quartz crystal microbalance evaluation that amelogenin is easy to adsorb onto the nano-modified titanium surface as a coating. Scanning electron microscopy, scanning probe microscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy analyses confirmed that amelogenin coated the nano-modified titanium surface following alkali-treatment. In vitro evaluation using rat bone marrow and periodontal ligament cells revealed that the initial adhesion of both cell types and the induction of hard tissue differentiation such as cementum were improved by amelogenin coating. Additionally, the formation of new bone in implanted surrounding tissues was observed in in vivo evaluation using rat femurs. Together, these results suggest that this material may serve as a new implant material with the potential to play a major role in the advancement of clinical dentistry.
- Subjects :
- Bone Regeneration
02 engineering and technology
amelogenin
nanostructure
host-implant interaction
tissue regeneration
lcsh:Chemistry
0302 clinical medicine
Coating
Coated Materials, Biocompatible
X-Ray Diffraction
Spectroscopy, Fourier Transform Infrared
lcsh:QH301-705.5
Spectroscopy
Cells, Cultured
Titanium
General Medicine
Adhesion
021001 nanoscience & nanotechnology
Computer Science Applications
medicine.anatomical_structure
0210 nano-technology
Materials science
Surface Properties
chemistry.chemical_element
Bone Marrow Cells
engineering.material
Catalysis
Article
Bone and Bones
Cell Line
Inorganic Chemistry
03 medical and health sciences
Dental Materials
medicine
Cell Adhesion
Periodontal fiber
Animals
Cementum
Physical and Theoretical Chemistry
Molecular Biology
Dental Implants
Organic Chemistry
technology, industry, and agriculture
030206 dentistry
Quartz crystal microbalance
Rats
chemistry
lcsh:Biology (General)
lcsh:QD1-999
engineering
Microscopy, Electron, Scanning
Implant
Amelogenin
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 19
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....6505c1bcdea0577c4025a1994d3a4c9c