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Semi-automated shear stress measurements in developing embryonic hearts

Authors :
Maryse Lapierre-Landry
Michael W. Jenkins
Shi Gu
Sahar Elahi
Andrew M. Rollins
Brecken J. Blackburn
Source :
Biomed Opt Express
Publication Year :
2020
Publisher :
Optica Publishing Group, 2020.

Abstract

Blood-induced shear stress influences gene expression. Abnormal shear stress patterns on the endocardium of the early-stage heart tube can lead to congenital heart defects. To have a better understanding of these mechanisms, it is essential to include shear stress measurements in longitudinal cohort studies of cardiac development. Previously reported approaches are computationally expensive and nonpractical when assessing many animals. Here, we introduce a new approach to estimate shear stress that does not rely on recording 4D image sets and extensive post processing. Our method uses two adjacent optical coherence tomography frames (B-scans) where lumen geometry and flow direction are determined from the structural data and the velocity is measured from the Doppler OCT signal. We validated our shear stress estimate by flow phantom experiments and applied it to live quail embryo hearts where observed shear stress patterns were similar to previous studies.

Details

ISSN :
21567085
Volume :
11
Database :
OpenAIRE
Journal :
Biomedical Optics Express
Accession number :
edsair.doi.dedup.....64d3acbc7509995269ad7b6578556c64
Full Text :
https://doi.org/10.1364/boe.395952