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Nano illumination microscopy: a technique based on scanning with an array of individually addressable nanoLEDs
- Source :
- Dipòsit Digital de la UB, Universidad de Barcelona, Optics Express
- Publication Year :
- 2020
-
Abstract
- In lensless microscopy, spatial resolution is usually provided by the pixel density of current digital cameras, which are reaching a hard-to-surpass pixel size / resolution limit over 1 µm. As an alternative, the dependence of the resolving power can be moved from the detector to the light sources, offering a new kind of lensless microscopy setups. The use of continuously scaled-down Light-Emitting Diode (LED) arrays to scan the sample allows resolutions on order of the LED size, giving rise to compact and low-cost microscopes without mechanical scanners or optical accessories. In this paper, we present the operation principle of this new approach to lensless microscopy, with simulations that demonstrate the possibility to use it for super-resolution, as well as a first prototype. This proof-of-concept setup integrates an 8 × 8 array of LEDs, each 5 × 5 μm2pixel size and 10 μmpitch, and an optical detector. We characterize the system using Electron-Beam Lithography (EBL) pattern. Our prototype validates the imaging principle and opens the way to improve resolution by further miniaturizing the light sources. © 2020 Optical Society of America under the terms of theOSA Open Access Publishing Agreement
- Subjects :
- Microscope
Materials science
Microscopis
nanoLEDs
CMOS SPAD
02 engineering and technology
01 natural sciences
Microscopes
law.invention
010309 optics
Optics
law
0103 physical sciences
Microscopy
Lithography
Image resolution
Pixel
business.industry
Detector
Resolution (electron density)
021001 nanoscience & nanotechnology
Sample (graphics)
Atomic and Molecular Physics, and Optics
Microscòpia
microscopy
0210 nano-technology
business
LED array
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 28
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Optics express
- Accession number :
- edsair.doi.dedup.....64d3037c10158c8acef11d586d9a54f9