Finger-Based Printed Sensors Integrated on a Glove for On-Site Screening Of Pseudomonas aeruginosa Virulence Factors

Early screening of clinically relevant pathogens in the environment is a highly desirable goal in clinical care, providing precious information that will improve patient-care outcomes. In this work, a glove-based electrochemical sensor has been designed for point-of-use screening of Pseudomonas aeruginosa’s virulence factors. The methodology used for the elaboration of the fabric platform relied on printing the conductive inks on the index and middle fingers of the glove, with the goal of screening pyocyanin and pyoverdine targets. The analytical signatures of the analytes were recorded in about 4 min, via the rapid and selective square-wave-voltammetry technique. Finger-based sensors display good performance and discrimination between the targets and potential interferences, along with good reproducibility. The sensors featured linearity over the 0.01–0.1 μM range for pyocyanin and 5–50 μM range for pyoverdine, with sensitivities of 2.51 μA/μM for pyocyanin and 1.09 nA/μM for pyoverdine (R2 = 0.990 and 0...