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Constitutive expression of p53 protein in enriched normal human marrow blast cell populations [see comments]
- Source :
- Blood. 79:1982-1986
- Publication Year :
- 1992
- Publisher :
- American Society of Hematology, 1992.
-
Abstract
- Previous studies by others using metabolic labeling, cell lysis, and immunoprecipitation have reported elevated levels of p53 protein in blast cells derived from patients with acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML), whereas p53 protein was not detected in normal light-density bone marrow cells. In this report, using the same detection methods, we confirm the negligible expression of p53 protein in normal light density marrow cells. However, we find clearly significant levels of p53 protein expression in enriched normal human marrow blast populations. Furthermore, using a panel of p53 specific monoclonal antibodies, we find the p53 protein constitutively synthesized by normal marrow blasts has the immunologic phenotype identified by PAb240 that reportedly recognizes a common conformational- dependent epitope on mutant p53. We have also found that the p53 immunologic subclass identified by PAb240 exists in normal human circulating lymphocytes either resting, serum starved, or PHA activated. In summary, it is clear that (1) normal marrow blast populations provide the appropriate control for assessing the levels of p53 protein expression in leukemic blast cells; and (2) PAb240 cannot be used to distinguish p53 mutated at the DNA level from normal p53 in fresh human hematopoietic cells.
- Subjects :
- medicine.medical_specialty
Hematology
Acute myeloblastic leukemia
medicine.drug_class
Immunoprecipitation
Immunology
Cell Biology
Biology
Monoclonal antibody
medicine.disease
Molecular biology
Biochemistry
Phenotype
Epitope
Haematopoiesis
medicine.anatomical_structure
Internal medicine
Precursor cell
medicine
Cancer research
Bone marrow
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....64d05291ebb75c041bfb6e86fc6f0e8f