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A plant diterpene counteracts juvenile hormone-mediated gene regulation during Drosophila melanogaster larval development
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 7, p e0200706 (2018)
- Publication Year :
- 2018
- Publisher :
- Public Library of Science, 2018.
-
Abstract
- Many plant species possess compounds with juvenile hormone disruptor (JHD) activity. In some plant species, such activity has been attributed to diterpene secondary metabolites. Plant JHD diterpenes disrupt insect development by interfering with the juvenile hormone (JH)-mediated formation of JH receptor complexes. Here, we demonstrate that a plant extract and a diterpene from Lindera erythrocarpa (methyl lucidone) interfere with the formation of both methoprene-tolerant (Met)/Taiman and Germ cell-expressed (GCE)/Taiman heterodimer complexes in yeast two-hybrid assays in vitro. In addition to the in vitro JHD activity, the diterpene and the plant extract from L. erythrocarpa also disrupt the development of larvae and pupae in Drosophila melanogaster. Comparing the transcriptomes of juvenile hormone analog (JHA, methoprene)- and JHD (methyl lucidone)-fed wandering third-instar larvae revealed a large number of genes that were coregulated by JHA and JHD. Moreover, most (83%) of the genes that were repressed by methyl lucidone were significantly activated by methoprene, indicating that JHDs and JHAs have opposing effects on the transcriptional regulation of many JH-dependent genes. Gene ontology analysis also suggested that some of the genes activated-by-JHA/repressed-by-JHD play roles in spermatogenesis. Affymetrix microarray-based analysis indicated that the expression of genes activated-by-JHA/repressed-by-JHD was testis-specific. Together, these results suggest that JH is involved in testis-specific gene expression and that plant JHD diterpenes function as JH antagonists in such JHA-mediated gene regulation.
- Subjects :
- 0301 basic medicine
Life Cycles
lcsh:Medicine
Gene Expression
Transcriptome
chemistry.chemical_compound
Larvae
Gene expression
Transcriptional regulation
Medicine and Health Sciences
lcsh:Science
Regulation of gene expression
Multidisciplinary
biology
Organic Compounds
Drosophila Melanogaster
Gene Ontologies
Eukaryota
Gene Expression Regulation, Developmental
Animal Models
Genomics
Cell biology
Insects
Juvenile Hormones
Chemistry
Experimental Organism Systems
Larva
Physical Sciences
Drosophila
Drosophila melanogaster
Diterpenes
Research Article
Arthropoda
Methoprene
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Genetics
Animals
Gene Disruption
Gene Regulation
Gene
Nutrition
Ethanol
Plant Extracts
lcsh:R
fungi
Organic Chemistry
Organisms
Chemical Compounds
Biology and Life Sciences
Computational Biology
biology.organism_classification
Genome Analysis
Invertebrates
Lindera
Diet
030104 developmental biology
chemistry
Alcohols
Juvenile hormone
lcsh:Q
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....64b4fabf84bd35da073d4c85fe3346db