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Fibrous nonlinear elasticity enables positive mechanical feedback between cells and ECMs
- Source :
- Proceedings of the National Academy of Sciences. 113:14043-14048
- Publication Year :
- 2016
- Publisher :
- Proceedings of the National Academy of Sciences, 2016.
-
Abstract
- In native states, animal cells of many types are supported by a fibrous network that forms the main structural component of the ECM. Mechanical interactions between cells and the 3D ECM critically regulate cell function, including growth and migration. However, the physical mechanism that governs the cell interaction with fibrous 3D ECM is still not known. In this article, we present single-cell traction force measurements using breast tumor cells embedded within 3D collagen matrices. We recreate the breast tumor mechanical environment by controlling the microstructure and density of type I collagen matrices. Our results reveal a positive mechanical feedback loop: cells pulling on collagen locally align and stiffen the matrix, and stiffer matrices, in return, promote greater cell force generation and a stiffer cell body. Furthermore, cell force transmission distance increases with the degree of strain-induced fiber alignment and stiffening of the collagen matrices. These findings highlight the importance of the nonlinear elasticity of fibrous matrices in regulating cell–ECM interactions within a 3D context, and the cell force regulation principle that we uncover may contribute to the rapid mechanical tissue stiffening occurring in many diseases, including cancer and fibrosis.
- Subjects :
- 0301 basic medicine
Materials science
Cell
Protein Array Analysis
Breast Neoplasms
Context (language use)
Cell Communication
02 engineering and technology
03 medical and health sciences
Matrix (mathematics)
Fibrosis
Cell Line, Tumor
medicine
Humans
Microscopy, Confocal
Multidisciplinary
Tractive force
Biological Sciences
021001 nanoscience & nanotechnology
medicine.disease
Elasticity
Biomechanical Phenomena
Extracellular Matrix
Stiffening
030104 developmental biology
medicine.anatomical_structure
Biophysics
Collagen
0210 nano-technology
Mechanoreceptors
Nonlinear elasticity
Type I collagen
Biomedical engineering
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....64b24f181caab9807b5137e6acaac8ae