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Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway

Authors :
Zhe Bo
Haiyi Zhu
Hongyan Gao
Yi Ren
Qin Wang
Ling Luo
Source :
Biomedicine & Pharmacotherapy, Vol 110, Iss, Pp 685-691 (2019)
Publication Year :
2019
Publisher :
Elsevier BV, 2019.

Abstract

Objective: Salvanic Acid B (Sal B) was proved to show significant effect against fibrosis and myocardial injury. This study aimed to investigate the protective effects and the mechanisms of Sal B on myocardial fibrosis. Methods: The mice were randomly assigned to five groups: control group, model group, positive group, low-dose group, high-dose group. Hematoxylin-Eosin (HE) staining and Masson staining were used to assess the myocardial physiological changes and measure the myocardial fibrosis area. Expression of transforming growth factor-beta (TGF-β), drosophila mothers against decapentaplegic (Smad)2, Smad3 and Smad7 were analyzed by immunohistochemistry and real-time PCR. On the other hand, mouse cardiac fibroblasts (CFs) cells were co-treated with 20 ng/mL TGF-β1 and different concentrations of Sal B (5, 10, and 20 ng/mL) for 24 h. The cells morphology changes were assessed under a microscope, and the protein expressions induced by TGF-β1 were detected by Western blot. Results: Compared with the model group, myocardial collagen fibers decreased obviously with Sal B treatment (p

Details

ISSN :
07533322
Volume :
110
Database :
OpenAIRE
Journal :
Biomedicine & Pharmacotherapy
Accession number :
edsair.doi.dedup.....64b009dfde2b6becd4bf2f06f0e5169f