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Shear Stress Increases ICAM-1 and Decreases VCAM-1 and E-selectin Expressions Induced by Tumor Necrosis Factor-α in Endothelial Cells
- Source :
- Arteriosclerosis, Thrombosis, and Vascular Biology. 24:73-79
- Publication Year :
- 2004
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2004.
-
Abstract
- Objective—Vascular endothelial cells (ECs) are subjected to shear stress and cytokine stimulation. We studied the interplay between shear stress and cytokine in modulating the expression of adhesion molecule genes in ECs.Methods and Results—Shear stress (20 dynes/cm2) was applied to ECs prior to and/or following the addition of tumor necrosis factor (TNF)-α. Shear stress increased the TNF-α–induced expression of intercellular adhesion molecule-1 (ICAM-1) at both mRNA and surface protein levels, but decreased the TNF-α–induced expression of vascular adhesion molecule-1 (VCAM-1) and E-selectin. Transfection studies using promoter reporter gene constructs of ICAM-1, VCAM-1, and E-selectin demonstrated that these shear stress modulations of gene expression occur at the transcriptional levels. After 24-hour preshearing followed by 1 hour of static incubation, the effect of preshearing on TNF-α–induced ICAM-1 mRNA expression vanished. The recovery of the TNF-α–induced VCAM-1 and E-selectin mRNA expressions following preshearing, however, required a static incubation time of >6 hours (complete recovery at 24 hours). Pre- and postshearing caused a reduction in the nuclear factor-κB-DNA binding activity induced by TNF-α in the EC nucleus.Conclusions—Our findings suggest that shear stress plays differential roles in modulating the TNF-α–induced expressions of ICAM-1 versus VCAM-1 and E-selectin genes in ECs.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Biology
chemistry.chemical_compound
Internal medicine
Gene expression
E-selectin
medicine
Shear stress
Humans
VCAM-1
Cells, Cultured
Cell Nucleus
ICAM-1
Tumor Necrosis Factor-alpha
NF-kappa B
Endothelial Cells
DNA
Cell biology
Endothelial stem cell
Cytokine
Endocrinology
Gene Expression Regulation
chemistry
biology.protein
Endothelium, Vascular
Stress, Mechanical
E-Selectin
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....64a396d81b37f2a38e57972b2b8dce96
- Full Text :
- https://doi.org/10.1161/01.atv.0000106321.63667.24