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Molecular evidence that follicle development is accelerated in vitro compared to in vivo

Authors :
D. Royère
F. Guerif
Philippe Monget
Yann Locatelli
Peggy Jarrier
Agnès Bonnet
Virginie Maillard
Véronique Cadoret
Danielle Monniaux
Cynthia Frapsauce
Physiologie de la reproduction et des comportements [Nouzilly] (PRC)
Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Med & Biol Reprod CECOS
CHU Bretonneau
Génétique Physiologie et Systèmes d'Elevage (GenPhySE )
École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT)
Institut National Polytechnique (Toulouse) (Toulouse INP)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA)
Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]
Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Source :
Reproduction, Reproduction, BioScientifica, 2017, 153 (5), pp.493-508. ⟨10.1530/REP-16-0627⟩
Publication Year :
2017
Publisher :
HAL CCSD, 2017.

Abstract

In this study, we systematically compared the morphological, functional and molecular characteristics of granulosa cells and oocytes obtained by a three-dimensionalin vitromodel of ovine ovarian follicular growth with those of follicles recoveredin vivo. Preantral follicles of 200 µm diameter were recovered and cultured up to 950 µm over a 20-day period. Compared within vivofollicles, thein vitroculture conditions maintained follicle survival, with no difference in the rate of atresia. However, thein vitroconditions induced a slight decrease in oocyte growth rate, delayed antrum formation and increased granulosa cell proliferation rate, accompanied by an increase and decrease inCCND2andCDKN1AmRNA expression respectively. These changes were associated with advanced granulosa cell differentiation in early antral follicles larger than 400 µm diameter, regardless of the presence or absence of FSH, as indicated by an increase in estradiol secretion, together with decreased AMH secretion and expression, as well as increased expression ofGJA1,CYP19A1,ESR1,ESR2,FSHR,INHA,INHBA,INHBBandFST. There was a decrease in the expression of oocyte-specific molecular markersGJA4,KIT,ZP3,WEE2andBMP15 in vitrocompared to thatin vivo. Moreover, a higher percentage of the oocytes recovered from cultured follicles 550 to 950 µm in diameter was able to reach the metaphase II meiosis stage. Overall, thisin vitromodel of ovarian follicle development is characterized by accelerated follicular maturation, associated with improved developmental competence of the oocyte, compared to follicles recoveredin vivo.

Details

Language :
English
ISSN :
14701626 and 17417899
Database :
OpenAIRE
Journal :
Reproduction, Reproduction, BioScientifica, 2017, 153 (5), pp.493-508. ⟨10.1530/REP-16-0627⟩
Accession number :
edsair.doi.dedup.....649cb97ed31b8935b4dd3f3e28fe1988
Full Text :
https://doi.org/10.1530/REP-16-0627⟩