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Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary
- Source :
- Viruses, Volume 13, Issue 1, Viruses, Vol 13, Iss 66, p 66 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Most picornaviruses of the family Picornaviridae are relatively well known, but there are certain &ldquo<br />neglected&rdquo<br />genera like Bopivirus, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and cre-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species Bopivirus A, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species &ldquo<br />Bopivirus B&rdquo<br />in the genus Bopivirus. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among &lt<br />12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine&ndash<br />caprine interspecies transmission of certain bopiviruses.
- Subjects :
- 0301 basic medicine
Picornavirus
030106 microbiology
Picornaviridae
lcsh:QR1-502
RT-PCR
Genome
lcsh:Microbiology
03 medical and health sciences
Monophyly
bopivirus
Phylogenetics
IRES
Virology
Genotype
caprine
Phylogenetic tree
biology
bovine
biology.organism_classification
livestock
phylogenetics
ovine
030104 developmental biology
Infectious Diseases
picornavirus
Capsid
epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....648c4ce65c7c9ade3b0be181968bfcd9
- Full Text :
- https://doi.org/10.3390/v13010066