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Evolutionary expression differences of creatine synthesis-related genes: Implications for skeletal muscle metabolism in fish
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group UK, 2019.
-
Abstract
- The creatine/phosphocreatine system is the principal energy buffer in mammals, but is scarcely documented in fish. We measured the gene expression of major enzymes of this system, glycine amidinotransferase (GATM), guanidinoacetate N-methyltransferase (GAMT) and muscle-type creatine kinase (CKM) in kidney, liver, and muscle tissues of fish and mammals. CKM was expressed strongly in the muscles of all examined species. In contrast, GATM and GAMT were strongly expressed in the muscle tissue of fish, but not of mammals. This indicates that creatine synthesis and usage are spatially separated in mammals, but not in fish, which is supported by RNA-Seq data of 25 species. Differences in amino acid metabolism along with methionine adenosyltransferase gene expression in muscle from fishes but not mammals further support a central metabolic role of muscle in fish, and hence different organization of the creatine/phosphocreatine biosynthesis system in higher and lower vertebrates.
- Subjects :
- 0301 basic medicine
Muscle tissue
Amidinotransferases
lcsh:Medicine
Biology
Creatine
Article
Phosphocreatine
Evolution, Molecular
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Gene expression
medicine
Animals
lcsh:Science
Muscle, Skeletal
Multidisciplinary
Sequence Analysis, RNA
Gene Expression Profiling
lcsh:R
Fishes
Skeletal muscle
Creatine Kinase, MM Form
Metabolism
Guanidinoacetate N-methyltransferase
030104 developmental biology
medicine.anatomical_structure
chemistry
Biochemistry
biology.protein
Creatine kinase
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....64866db9069d185e5739d46b875993ae