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Assignment of the configurations of the amino acids in peptidic siderophores
- Source :
- Journal of Chromatography A. 438:291-297
- Publication Year :
- 1988
- Publisher :
- Elsevier BV, 1988.
-
Abstract
- Pyoverdins and azotobactins contain beta-hydroxyaspartic acid, N delta-hydroxyornithine, citrulline and homoserine, in addition to the common protein amino acids. Configuration assignment of all of these was achieved by acid hydrolysis of the peptide, derivatization of the constituent amino acids to the N-pentafluoropropionyl amino acid esters and gas chromatographic separation of the stereoisomers on capillaries coated with Chirasil-Val. This approach is straightforward for the protein amino acids, but the less common amino acids are either partially degraded during acid hydrolysis or their derivatives exhibit unfavourable gas chromatographic properties. By judicious combination of partial and total hydrolysis and dual derivatization, these problems may be overcome.
- Subjects :
- Chromatography, Gas
Protein Conformation
Homoserine
Siderophores
Peptide
Iron Chelating Agents
Biochemistry
Gas Chromatography-Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Hydrolysis
Protein structure
Organic chemistry
Amino Acids
chemistry.chemical_classification
Chromatography
Organic Chemistry
Pigments, Biological
General Medicine
Xanthoproteic reaction
Photo-reactive amino acid analog
Amino acid
chemistry
Pseudomonas aeruginosa
Acid hydrolysis
Peptides
Oligopeptides
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 438
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....646c5988cc3acd9b573218d883c76664