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Target-Responsive DNA Hydrogel Mediated 'Stop-Flow' Microfluidic Paper-Based Analytic Device for Rapid, Portable and Visual Detection of Multiple Targets
- Source :
- Analytical Chemistry. 87:4275-4282
- Publication Year :
- 2015
- Publisher :
- American Chemical Society (ACS), 2015.
-
Abstract
- A versatile point-of-care assay platform was developed for simultaneous detection of multiple targets based on a microfluidic paper-based analytic device (μPAD) using a target-responsive hydrogel to mediate fluidic flow and signal readout. An aptamer-cross-linked hydrogel was used as a target-responsive flow regulator in the μPAD. In the absence of a target, the hydrogel is formed in the flow channel, stopping the flow in the μPAD and preventing the colored indicator from traveling to the final observation spot, thus yielding a "signal off" readout. In contrast, in the presence of a target, no hydrogel is formed because of the preferential interaction of target and aptamer. This allows free fluidic flow in the μPAD, carrying the indicator to the observation spot and producing a "signal on" readout. The device is inexpensive to fabricate, easy to use, and disposable after detection. Testing results can be obtained within 6 min by the naked eye via a simple loading operation without the need for any auxiliary equipment. Multiple targets, including cocaine, adenosine, and Pb(2+), can be detected simultaneously, even in complex biological matrices such as urine. The reported method offers simple, low cost, rapid, user-friendly, point-of-care testing, which will be useful in many applications.
- Subjects :
- Paper
Adenosine
Molecular Structure
Chemistry
business.industry
Point-of-Care Systems
Aptamer
Stop flow
Microfluidics
technology, industry, and agriculture
Nanotechnology
DNA
Paper based
Microfluidic Analytical Techniques
Signal
Hydrogel, Polyethylene Glycol Dimethacrylate
Analytical Chemistry
Organophosphorus Compounds
Visual detection
Cocaine
Lead
Optoelectronics
Fluidics
Naked eye
business
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....646c196736f43d5deef840c6e93a5f1a