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Effects of intrinsic metal ions of lentinan with different molecular weights from Lentinus edodes on the antioxidant capacity and activity against proliferation of cancer cells
- Source :
- International Journal of Biological Macromolecules. 120:73-81
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- In this study, effects of intrinsic metal ions in lentinan on its bioactivities were evaluated. Lentinan extracted from Lentinus edodes was separated to four fractions with different molecular weights with Sephadex G-200 gel chromatography, and intrinsic metal ions were removed by EDTA. The ferrous ion chelating capacity of ELWs was measured to estimate EDTA residue after extensive dialysis. Antioxidant and antitumor activities of the isolated lentinans (LWs and ELWs) were investigated in vitro and compared, respectively. The results indicated that all of the isolated lentinan contained a large amount of intrinsic metal ions and the fraction with the lowest Mw had highest metal content. After removing intrinsic metal ions, the polysaccharides showed lower ABTS and DPPH radicals scavenging capacity and the reduced inhibition of the proliferation of BXPC-3 and Hela cells. Our results indicated that the metal ions in lentinan had positive effects on its bioactivities.
- Subjects :
- 0301 basic medicine
DPPH
Metal ions in aqueous solution
Lentinan
Shiitake Mushrooms
Antineoplastic Agents
02 engineering and technology
Biochemistry
Antioxidants
Gel permeation chromatography
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
Neoplasms
Humans
Chelation
Molecular Biology
Cell Proliferation
ABTS
biology
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
030104 developmental biology
chemistry
Metals
Sephadex
Lentinus
0210 nano-technology
HeLa Cells
Nuclear chemistry
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....643e5727f0bba85523af00568eae8653