Back to Search
Start Over
Ultra-High-Resolution 3D Optical Coherence Tomography Reveals Inner Structures of Human Placenta-Derived Trophoblast Organoids
- Source :
- IEEE Transactions on Biomedical Engineering. 68:2368-2376
- Publication Year :
- 2021
- Publisher :
- Institute of Electrical and Electronics Engineers (IEEE), 2021.
-
Abstract
- Objective: 3D optical coherence tomography (OCT) is used for analyses of human placenta organoids in situ without sample preparation. Methods: The trophoblast organoids analyzed were derived from primary human trophoblast. In this study a custom made ultra-high-resolution spectral domain OCT system with uniform spatial and axial resolution of 2.48 μm in organoid tissue was used. The obtained OCT results align to differentiation status tested via quantitative polymerase chain reaction, Western blot analyses, immunohistochemistry, and immunofluorescence of histological sections. Results: 3D OCT enables a more detailed placenta organoid monitoring compared to brightfield microscopy. Inner architecture with light scattering “bridges” surrounding cavities were visualized and quantified in situ for the first time. The formation of these bridges and cavities is congruent to differentiated trophoblast organoids having developed syncytiotrophoblasts. Conclusion: Using 3D OCT in living placenta organoids is a fast tool to assess the differentiation status and resolve internal structures in situ , which is not possible with standard live cell imaging modality. Significance: Only recently human placenta-derived organoids were established, allowing to have a highly reproducible and stable in vitro model to investigate not only developmental but also physiological and pathophysiological processes during early pregnancy. To our knowledge, this work is the first to analyze living human placenta organoids using 3D OCT. Thereby, the rapid and especially non-endpoint OCT qualitative analyses align to the differentiation stage of organoids, which will aid future advancement in this field.
- Subjects :
- In situ
genetic structures
medicine.diagnostic_test
Chemistry
Placenta
Bright-field microscopy
Biomedical Engineering
Trophoblast
Cell Differentiation
Syncytiotrophoblasts
Trophoblasts
Cell biology
Organoids
medicine.anatomical_structure
Optical coherence tomography
Pregnancy
Live cell imaging
embryonic structures
Organoid
medicine
Humans
Female
Tomography, Optical Coherence
Subjects
Details
- ISSN :
- 15582531 and 00189294
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- IEEE Transactions on Biomedical Engineering
- Accession number :
- edsair.doi.dedup.....643c2bc75b4430144fbe6c63cb88af42