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The protective effects of ozone therapy in a rat model of acetaminophen-induced liver injury
- Source :
- Environmental Toxicology and Pharmacology. 34:81-86
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- a b s t r a c t Objectives: Acetaminophen (APAP) overdose may cause acute liver injury. Ozone therapy (OT) is shown to reduce inflammation and necrosis in several entities. Thus, we have designed this study to evaluate the efficacy of OT in a rat model of APAP-induced liver injury. Methods: Twenty-seven Sprague-Dawley rats were divided into three groups: sham, APAP and APAP+OT groups. In the APAP and the APAP+OT groups, liver injury was induced by oral administration of 1 g/kg APAP. The APAP+OT group received a single dose ozone/oxygen mixture (0.7 mg/kg) intraperitoneally 1 h after APAP administration. All animals were killed at 24 hour after APAP administration. Blood samples and liver tissues were harvested to determine liver injury and oxidative stress parameters. Liver tissues and blood samples were obtained for biochemical and histopathological analyses. Results: APAP administration caused necrosis in the liver after 24 h. The degrees of liver necrosis of the APAP group were higher than the other groups (in both p < 0.05, respectively). In the APAP+OT group, liver antioxidant enzymes activities were significantly higher than the APAP group (p < 0.05), but were lower than the sham group (p < 0.05). In the sham group, serum neopterin, a marker of cell-mediated immunity, concentrations (4.8 ± 1.2 nmol/L) were lower than the APAP (14.7 ± 1.4 nmol/L) and APAP+OT groups (7.5 ± 2.4 nmol/L) (in both p < 0.05, respectively). Conclusion: Our results showed that OT prevented liver necrosis in rats and reduced neopterin levels. These findings suggest that the use of OT as an adjuvant therapy which might improve the outcome in APAP induced liver injury.
- Subjects :
- Male
Necrosis
Health, Toxicology and Mutagenesis
Inflammation
Pharmacology
Protective Agents
Toxicology
medicine.disease_cause
Neopterin
Rats, Sprague-Dawley
chemistry.chemical_compound
Ozone
Oral administration
Malondialdehyde
Animals
Medicine
Aspartate Aminotransferases
Nitrites
Acetaminophen
Liver injury
Glutathione Peroxidase
Nitrates
Superoxide Dismutase
business.industry
digestive, oral, and skin physiology
Alanine Transaminase
General Medicine
Analgesics, Non-Narcotic
medicine.disease
Ozone therapy
Rats
Disease Models, Animal
Liver
chemistry
Anesthesia
Chemical and Drug Induced Liver Injury
medicine.symptom
business
Oxidative stress
medicine.drug
Subjects
Details
- ISSN :
- 13826689
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Environmental Toxicology and Pharmacology
- Accession number :
- edsair.doi.dedup.....6439667f7189518b0875a6729d0ca55a