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Low angle light scattering analysis: a novel quantitative method for functional characterization of human and murine platelet receptors
- Source :
- Clinical chemistry and laboratory medicine. 50(7)
- Publication Year :
- 2011
-
Abstract
- Background: Determinations of platelet receptor functions are indispensable diagnostic indicators of cardiovascular and hemostatic diseases including hereditary and acquired receptor defects and receptor responses to drugs. However, presently available techniques for assessing platelet function have some disadvantages, such as low sensitivity and the requirement of large sample sizes and unphysiologically high agonist concentrations. Our goal was to develop and initially characterize a new technique designed to quantitatively analyze platelet receptor activation and platelet function on the basis of measuring changes in low angle light scattering. Methods: We developed a novel technique based on low angle light scattering registering changes in light scattering at a range of different angles in platelet suspensions during activation. Results: The method proved to be highly sensitive for simultaneous real time detection of changes in size and shape of platelets during activation. Unlike commonly-used methods, the light scattering method could detect platelet shape change and aggregation in response to nanomolar concentrations of extracellular nucleotides. Furthermore, our results demonstrate that the advantages of the light scattering method make it a choice method for platelet receptor monitoring and for investigation of both murine and human platelets in disease models. Conclusions: Our data demonstrate the suitability and superiority of this new low angle light scattering method for comprehensive analyses of platelet receptors and functions. This highly sensitive, quantitative, and online detection of essential physiological, pathophysiological and pharmacological-response properties of human and mouse platelets is a significant improvement over conventional techniques.
- Subjects :
- Agonist
Blood Platelets
P2Y receptor
Shape change
Light
Platelet Aggregation
Platelet Function Tests
medicine.drug_class
Clinical Biochemistry
Analytical chemistry
030204 cardiovascular system & hematology
Light scattering
03 medical and health sciences
Mice
Thromboxane A2
0302 clinical medicine
Adenosine Triphosphate
Image Interpretation, Computer-Assisted
medicine
Animals
Humans
Scattering, Radiation
Platelet
Receptor
030304 developmental biology
aggregation
platelet
P2X1 receptor
P2Y receptors
0303 health sciences
Chemistry
Platelet-Rich Plasma
Receptors, Purinergic P2
Biochemistry (medical)
General Medicine
Middle Aged
Characterization (materials science)
Adenosine Diphosphate
Biophysics
Collagen
Function (biology)
Subjects
Details
- ISSN :
- 14374331
- Volume :
- 50
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Clinical chemistry and laboratory medicine
- Accession number :
- edsair.doi.dedup.....642170b6047e4b4fdbfd78642df68190