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Degradation of Methanethiol by Methylotrophic Methanogenic Archaea in a Lab-Scale Upflow Anaerobic Sludge Blanket Reactor
- Source :
- Applied and Environmental Microbiology 72 (2006) 12, Applied and Environmental Microbiology, 72(12), 7540-7547
- Publication Year :
- 2006
-
Abstract
- In a lab-scale upflow anaerobic sludge blanket reactor inoculated with granular sludge from a full-scale wastewater treatment plant treating paper mill wastewater, methanethiol (MT) was degraded at 30°C to H 2 S, CO 2 , and CH 4 . At a hydraulic retention time of 9 h, a maximum influent concentration of 6 mM MT was applied, corresponding to a volumetric loading rate of 16.5 mmol liter −1 day −1 . The archaeal community within the reactor was characterized by anaerobic culturing and denaturing gradient gel electrophoresis analysis, cloning, and sequencing of 16S rRNA genes and quantitative PCR. Initially, MT-fermenting methanogenic archaea related to members of the genus Methanolobus were enriched in the reactor. Later, they were outcompeted by Methanomethylovorans hollandica , which was detected in aggregates but not inside the granules that originated from the inoculum, the microbial composition of which remained fairly unchanged. Possibly other species within the Methanosarcinacaea also contributed to the fermentation of MT, but they were not enriched by serial dilution in liquid media. The archaeal community within the granules, which was dominated by Methanobacterium beijingense , did not change substantially during the reactor operation. Some of the species related to Methanomethylovorans hollandica were enriched by serial dilutions, but their growth rates were very low. Interestingly, the enrichments could be sustained only in the presence of MT and did not utilize any of the other typical substrates for methylotrophic methanogens, such as methanol, methyl amine, or dimethylsulfide.
- Subjects :
- Paper
Methanobacterium
waste-water
Hydraulic retention time
Methanogenesis
Molecular Sequence Data
Industrial Waste
Methanethiol
sulfate
digestion
Waste Disposal, Fluid
Applied Microbiology and Biotechnology
Microbiology
chemistry.chemical_compound
bioreactor
Bioreactors
Microbiologie
RNA, Ribosomal, 16S
Bioreactor
Anaerobiosis
Sulfhydryl Compounds
conversion
bacteria
dimethyl sulfide
WIMEK
Sewage
Ecology
biology
sediments
Methanosarcinaceae
Sequence Analysis, DNA
Physiology and Biotechnology
biology.organism_classification
communities
sulfur-compounds
Biodegradation, Environmental
chemistry
Environmental chemistry
Environmental Technology
Milieutechnologie
Laboratories
Temperature gradient gel electrophoresis
Food Science
Biotechnology
Archaea
Waste disposal
Subjects
Details
- Language :
- English
- ISSN :
- 00992240
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology 72 (2006) 12, Applied and Environmental Microbiology, 72(12), 7540-7547
- Accession number :
- edsair.doi.dedup.....6414c91bba8d2f487f30c8b01af5f9dc