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Functional Pentameric Formation via Coexpression of the Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Fusion Protein Subunit with a Neutralizing Epitope of ApxIIA Exotoxin Improves the Mucosal Immunogenicity and Protection against Challenge by Actinobacillus pleuropneumoniae
- Source :
- Clinical and Vaccine Immunology. 18:2168-2177
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- A coexpression strategy in Saccharomyces cerevisiae using episomal and integrative vectors for the Escherichia coli heat-labile enterotoxin B subunit (LTB) and a fusion protein of an ApxIIA toxin epitope produced by Actinobacillus pleuropneumoniae coupled to LTB, respectively, was adapted for the hetero-oligomerization of LTB and the LTB fusion construct. Enzyme-linked immunosorbent assay (ELISA) with GM1 ganglioside indicated that the LTB fusion construct, along with LTB, was oligomerized to make the functional heteropentameric form, which can bind to receptors on the mucosal epithelium. The antigen-specific antibody titer of mice orally administered antigen was increased when using recombinant yeast coexpressing the pentameric form instead of recombinant yeast expressing either the LTB fusion form or antigen alone. Better protection against challenge infection with A. pleuropneumoniae was also observed for coexpression in recombinant yeast compared with others. The present study clearly indicated that the coexpression strategy enabled the LTB fusion construct to participate in the pentameric formation, resulting in an improved induction of systemic and mucosal immune responses.
- Subjects :
- Microbiology (medical)
Recombinant Fusion Proteins
Bacterial Toxins
Clinical Biochemistry
Immunology
Saccharomyces cerevisiae
Enterotoxin
Biology
Heat-labile enterotoxin
medicine.disease_cause
Epitope
Microbiology
Enterotoxins
Epitopes
Hemolysin Proteins
Mice
Actinobacillus Infections
Bacterial Proteins
Antigen
Escherichia coli
medicine
Administration, Mucosal
Animals
Immunology and Allergy
Actinobacillus pleuropneumoniae
Escherichia coli Vaccines
Escherichia coli Proteins
Immunogenicity
Vaccine Research
biology.organism_classification
Antibodies, Bacterial
Survival Analysis
Fusion protein
Disease Models, Animal
Protein Subunits
Protein Multimerization
Exotoxin
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology
- Accession number :
- edsair.doi.dedup.....640a9d9bc3245b3463fa97e1c0967633