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Increased Production of Food-Grade <scp>d</scp>-Tagatose from <scp>d</scp>-Galactose by Permeabilized and Immobilized Cells of Corynebacterium glutamicum, a GRAS Host, Expressing <scp>d</scp>-Galactose Isomerase from Geobacillus thermodenitrificans
- Source :
- Journal of Agricultural and Food Chemistry. 64:8146-8153
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- The generally recognized as safe microorganism Corynebacterium glutamicum expressing Geobacillus thermodenitrificans d-galactose isomerase (d-GaI) was an efficient host for the production of d-tagatose, a functional sweetener. The d-tagatose production at 500 g/L d-galactose by the host was 1.4-fold higher than that by Escherichia coli expressing d-GaI. The d-tagatose-producing activity of permeabilized C. glutamicum (PCG) cells treated with 1% (w/v) Triton X-100 was 2.1-fold higher than that of untreated cells. Permeabilized and immobilized C. glutamicum (PICG) cells in 3% (w/v) alginate showed a 3.1-fold longer half-life at 50 °C and 3.1-fold higher total d-tagatose concentration in repeated batch reactions than PCG cells. PICG cells, which produced 165 g/L d-tagatose after 3 h, with a conversion of 55% (w/w) and a productivity of 55 g/L/h, showed significantly higher d-tagatose productivity than that reported for other cells. Thus, d-tagatose production by PICG cells may be an economical process to produce food-grade d-tagatose.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Geobacillus thermodenitrificans
Octoxynol
Microorganism
Isomerase
Biology
medicine.disease_cause
01 natural sciences
Permeability
Corynebacterium glutamicum
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Generally recognized as safe
medicine
Escherichia coli
Hexoses
Host (biology)
Temperature
Galactose
Geobacillus
General Chemistry
Cells, Immobilized
Hydrogen-Ion Concentration
Recombinant Proteins
Culture Media
030104 developmental biology
chemistry
Biochemistry
Sweetening Agents
General Agricultural and Biological Sciences
Biotechnology
Half-Life
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....63f6a2c46333b8175ae1e20ffe2391b9