Back to Search
Start Over
Developmental Neurotoxicity of Methamidophos in the Embryo-Larval Stages of Zebrafish
- Source :
- International Journal of Environmental Research and Public Health, International Journal of Environmental Research and Public Health; Volume 14; Issue 1; Pages: 23, International Journal of Environmental Research and Public Health, Vol 14, Iss 1, p 23 (2016)
- Publication Year :
- 2016
- Publisher :
- MDPI, 2016.
-
Abstract
- Methamidophos is a representative organophosphate insecticide. The knowledge of its developmental neurotoxicity is limited, especially for zebrafish in the early stages of their life. Four hour post-fertilization (hpf) zebrafish embryos were exposed to several environmentally relevant concentrations of methamidophos (0, 25, and 500 μg/L) for up to 72 hpf. Locomotor behavior was then studied in the zebrafish larvae at this timepoint. Acridine orange (AO) staining was carried out in the zebrafish larvae, and the mRNA levels of genes associated with neural development (mbp and syn2a) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The number of escape responders for mechanical stimulation was significantly decreased in exposed groups. AO staining showed noticeable signs of apoptosis mainly in the brain. In addition, the mRNA levels of mbp and syn2a were both significantly down-regulated in exposed groups. Our study provides the first evidence that methamidophos exposure can cause developmental neurotoxicity in the early stages of zebrafish life, which may be caused by the effect of methamidophos on neurodevelopmental genes and the activation of cell apoptosis in the brain.
- Subjects :
- 0301 basic medicine
Insecticides
animal structures
Embryo, Nonmammalian
Health, Toxicology and Mutagenesis
lcsh:Medicine
Stimulation
Apoptosis
010501 environmental sciences
01 natural sciences
Article
Andrology
03 medical and health sciences
chemistry.chemical_compound
Organophosphorus Compounds
Animals
RNA, Messenger
Zebrafish
0105 earth and related environmental sciences
methamidophos
developmental neurotoxicity
zebrafish
toxicology
biology
Methamidophos
lcsh:R
Organophosphate
Acridine orange
fungi
Public Health, Environmental and Occupational Health
Gene Expression Regulation, Developmental
Embryo
Organothiophosphorus Compounds
Anatomy
biology.organism_classification
030104 developmental biology
chemistry
Larva
embryonic structures
Neurotoxicity Syndromes
Neural development
Subjects
Details
- Language :
- English
- ISSN :
- 16604601 and 16617827
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....63f0768f284fe34fe7c9ff0b8ed20ebf