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Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice
- Source :
- Experimental dermatology. 27(4)
- Publication Year :
- 2017
-
Abstract
- Atopic dermatitis (AD)-like dermatitis can be induced by repeated topical application of an ointment containing Dermatophagoides farinae body (Dfb) extract in NC/Nga mice. This AD-like murine model also exhibits a biphasic increase in the number of scratching behaviour after topical application of Dfb ointment. In this study, we investigated the possible mechanisms underlying the scratching behaviour in each phase. An increase in the content of mast cell-derived mediators such as histamine and 5-hydroxytryptamine in the lesional skin and increased vascular permeability were observed in the early phase after the Dfb ointment application. Chlorpheniramine (H1 receptor antagonist) and cromoglycate (mast cell stabilizer) reduced the scratching behaviour in the early phase but not that in the later phase. Furthermore, the content of various endogenous pruritogens such as interleukin-31 and thymic stromal lymphopoietin in the lesional skin was increased 1 or 24 hours after the Dfb ointment application. Elevated expression of proteinase-activated receptor-2 (PAR-2) was also observed in the epidermis. Finally, gabexate (serine protease inhibitor) reduced the scratching behaviour in both phases, and anti-PAR2 antibody also showed a tendency to reduce both scratching behaviours. These findings suggest that immediate-type allergic reactions caused by mast cell degranulation and PAR-2 activation by proteases are involved in the scratching behaviour in this AD-like model.
- Subjects :
- 0301 basic medicine
Pharmacology
Biochemistry
Ointments
030207 dermatology & venereal diseases
chemistry.chemical_compound
Mice
0302 clinical medicine
Gabexate
Mast cell stabilizer
Mast Cells
Behavior, Animal
Dermatophagoides farinae
Degranulation
Atopic dermatitis
Cytokines
Female
Histamine
Proteases
Chlorpheniramine
Serotonin
Thymic stromal lymphopoietin
Serine Proteinase Inhibitors
medicine.drug_class
Dermatology
Complex Mixtures
Antibodies
Dermatitis, Atopic
Capillary Permeability
03 medical and health sciences
Thymic Stromal Lymphopoietin
Cromolyn Sodium
medicine
Hypersensitivity
Animals
Immunologic Factors
Receptor, PAR-2
Molecular Biology
Interleukins
Pruritus
Antipruritics
Scratching
medicine.disease
eye diseases
Disease Models, Animal
030104 developmental biology
chemistry
Epidermis
Subjects
Details
- ISSN :
- 16000625
- Volume :
- 27
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Experimental dermatology
- Accession number :
- edsair.doi.dedup.....63c6b533e7c6a0d19fd596d2b06d10e3