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Evidence for cell surface association of CD2 and LFA-1 (CD11a/CD18) on T lymphocytes
- Source :
- European Journal of Immunology. 24:450-457
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- Previous studies have reported an association of the cell surface adhesion molecule CD2 with the T cell receptor and with CD45 on mouse and human T lymphocytes. In this study the association of CD2 with cell surface molecules was investigated using cell surface biotinylation of T lymphocytes, coupled with immunoprecipitation using two CD2-specific monoclonal antibodies (mAb) (RM2-5 and 12-15) and analysis by SDS-PAGE. Although both CD2 mAb immunoprecipitated CD2 from lysates of murine lymphocytes, it was found that mAb 12-15, but not RM2-5, co-precipitated two other molecules of 95 and 180 kDa. Subsequent studies revealed that the 95- and 180-kDa molecules were associated with a subspecies of CD2 (approximately 5%) on thymocytes, the antigen-specific T cell line D10, and splenic T cells but not B cells. Two lines of evidence were obtained consistent with the 95- and 180-kDa molecules being the beta and alpha chains of LFA-1. Firstly, an analysis of 12-15 mAb immunoprecipitates on 4-12% gels under reducing and nonreducing conditions shows that the 95- and 180-kDa molecules have a molecular weight and migration pattern identical to LFA-1. Secondly, depletion of LFA-1 from lysates with LFA-1 mAb abolished the ability of CD2 mAb 12-15 to co-precipitate the 95- and 180-kDa molecules, thereby identifying these as the beta and alpha chains of mouse LFA-1, respectively. These results provide evidence for the first time for an association of LFA-1 and CD2 on mouse T lymphocytes, and suggest that the association occurs with an immunologically distinct subspecies of CD2 molecules.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte
Macromolecular Substances
medicine.drug_class
Immunoprecipitation
T-Lymphocytes
T cell
Immunology
Cell
CD2 Antigens
Biology
Monoclonal antibody
Mice
medicine
Animals
Immunology and Allergy
Lymphocyte function-associated antigen 1
Receptors, Immunologic
B-Lymphocytes
T-cell receptor
Antibodies, Monoclonal
T lymphocyte
Precipitin Tests
Molecular biology
Lymphocyte Function-Associated Antigen-1
Molecular Weight
medicine.anatomical_structure
Biotinylation
Antigens, Surface
Mice, Inbred CBA
Female
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....639b518d631cc1c4710a5a1479d58577