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Manipulation of Single-Stranded DNA by Using an Artificial Site-Selective DNA Cutter Composed of Cerium(IV)/EDTA and Phosphonate-Oligonucleotide Conjugates
- Source :
- Chemistry - An Asian Journal. 6:2407-2411
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- An artificial site-selective DNA cutter to hydrolyze single-stranded DNA at a desired site was prepared from Ce(IV)/ethylenediamintetraacetic acid (EDTA) and two ethylenediamine-N,N,N',N'-tetrakis(methylenephosphonic acid)-oligonucleotide conjugates. By using this cutter, the sense strand of a blue fluorescent protein (BFP) gene was selectively cut at a predetermined site in the chromophore-coding region. The upstream fragment obtained by the site-selective scission was ligated with the downstream fragment of the closely related green fluorescent protein (GFP) gene so that the 5'- and 3'-end portions of the chromophore came from the BFP fragment and the GFP fragment, respectively. The recombinant gene was successfully expressed in E. coli and the chimeric chromophore emitted green fluorescence as expected.
- Subjects :
- Stereochemistry
Oligonucleotides
Organophosphonates
DNA, Single-Stranded
Biochemistry
law.invention
Green fluorescent protein
chemistry.chemical_compound
law
Escherichia coli
Gene
Edetic Acid
Oligonucleotide
Hydrolysis
Organic Chemistry
Cerium
General Chemistry
Phosphonate
Fluorescence
Molecular biology
Recombinant Proteins
Luminescent Proteins
chemistry
Sense strand
Recombinant DNA
DNA
Subjects
Details
- ISSN :
- 18614728
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Chemistry - An Asian Journal
- Accession number :
- edsair.doi.dedup.....63767bf377bda354425a3c20534aebc3