Back to Search
Start Over
Induction of human interleukin 1 by bacterial and synthetic lipid A
- Source :
- European journal of immunology. 16(10)
- Publication Year :
- 1986
-
Abstract
- Lipopolysaccharides (LPS) are well known inducers of interleukin 1 (IL 1). Here we show that synthetic heptaacyl Salmonella minnesota (compound 516) and synthetic E. coli type (compound 506) lipid A, as well as monodephospho part structures thereof, are able to induce IL 1 production in human mononuclear cells. The 4'-monodephospho part structure (compound 505) was found to be the most active IL 1 inducer as compared to compound 506 and the 1-monodephospho part structure (compound 504). Synthetic lipid A precursor Ia, lacking nonhydroxylated fatty acids (compound 406), and its 1- or 4'-monodephospho part structures (compounds 404 and 405) did not induce IL 1 production. IL 2 is not produced during stimulation with the synthetic compounds. IL 1 activity was partially heat labile and could be inhibited by a rabbit antiserum against human leukocytic pyrogen. These results show that the lipid A component of LPS is active in inducing IL 1 and that the presence of 3-acyloxyacyl residues is necessary for IL 1 induction.
- Subjects :
- Salmonella minnesota
Hot Temperature
Time Factors
biology
Lipopolysaccharide
Immunology
Interleukin
Stimulation
biology.organism_classification
Blood Physiological Phenomena
Peripheral blood mononuclear cell
Lipid A
chemistry.chemical_compound
Structure-Activity Relationship
chemistry
Biochemistry
Immunology and Allergy
Humans
Interleukin-2
Inducer
Bacteria
Interleukin-1
Subjects
Details
- ISSN :
- 00142980
- Volume :
- 16
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- European journal of immunology
- Accession number :
- edsair.doi.dedup.....636d3ee51170afc4ade7cffcc9090c93