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Methodology for computing white matter nerve fiber orientation in human histological slices
- Source :
- Journal of Neuroscience Methods. 261:75-84
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Background The gold standard for mapping nerve fiber orientation in white matter of the human brain is histological analysis through biopsy. Such mappings are a crucial step in validating non-invasive techniques for assessing nerve fiber orientation in the human brain by using diffusion MRI. However, the manual extraction of nerve fiber directions of histological slices is tedious, time consuming, and prone to human error. New method The presented semi-automated algorithm first creates a binary-segmented mask of the nerve fibers in the histological image, and then extracts an estimate of average directionality of nerve fibers through a Fourier-domain analysis of the masked image. It also generates an uncertainty level for its estimate of average directionality. Results and comparison with existing methods The average orientations of the semi-automatic method were first compared to a qualitative expert opinion based on visual inspection of nerve fibers. A weighted RMS difference between the expert estimate and the algorithmically determined angle (weighted by expert's confidence in his estimate) was 15.4°, dropping to 9.9° when only cases with an expert confidence level of greater than 50% were included. The algorithmically determined angles were then compared with angles extracted using a manual segmentation technique, yielding an RMS difference of 11.2°. Conclusion The presented semi-automated method is in good agreement with both qualitative and quantitative manual expert-based approaches for estimating directionality of nerve fibers in white matter from images of stained histological slices of the human brain.
- Subjects :
- Automated
Histology
Computer science
Image Processing
Nerve fibers orientation
Nerve fibers
Bioengineering
Nerve fiber
Pattern Recognition
Nerve Fibers, Myelinated
Hippocampus
Article
Pattern Recognition, Automated
030218 nuclear medicine & medical imaging
White matter
03 medical and health sciences
Computer-Assisted
0302 clinical medicine
Average orientation
Image Processing, Computer-Assisted
medicine
Humans
Psychology
Neurology & Neurosurgery
Fourier Analysis
Orientation (computer vision)
General Neuroscience
Histological Techniques
Neurosciences
Gold standard (test)
White Matter
Manual extraction
medicine.anatomical_structure
Orientation map
Expert opinion
Fourier transform
Myelinated
Biomedical Imaging
Fiber tracking
Cognitive Sciences
Manual segmentation
Neuroscience
Algorithms
030217 neurology & neurosurgery
Biomedical engineering
Diffusion MRI
Subjects
Details
- ISSN :
- 01650270
- Volume :
- 261
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Methods
- Accession number :
- edsair.doi.dedup.....6369f0fdd849c187f64787fe16da36ba