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Genomic organization and expression of mouse Tpt1 gene☆
- Source :
- Genomics, Genomics, Elsevier, 2003, 81 (6), pp.570-578. ⟨10.1016/s0888-7543(03)00047-8⟩
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- International audience; The translationally controlled tumor protein (TCTP), also known as histamine-releasing factor (HRF), is encoded by a gene (Tpt1) that is highly conserved throughout phylogeny. TCTP is implicated in cell growth, acute allergic response, and apoptosis. In the present study, seven putative Tpt1 genes with different chromosomal localizations were identified in the mouse genome. In six of them, analysis of the 5' and 3' untranslated regions revealed the presence of flanking direct repeats and residual poly(A) tails typical of pseudogenes. Only three of the seven genes can produce a protein of the expected molecular weight. We isolated the genomic DNA of these three genes to analyze their sequence, genomic organization, and in vitro promoter activity. We found that mouse Tpt1 is localized on chromosome 14 with a canonical intron-exon organization, a functional promoter, and only one transcript that is ubiquitously expressed in all tissues.
- Subjects :
- Saccharomyces cerevisiae Proteins
[SDV]Life Sciences [q-bio]
Pseudogene
Molecular Sequence Data
Biology
Genome
Chromosomes
Mice
03 medical and health sciences
Translationally-controlled tumor protein
Biomarkers, Tumor
Genetics
Animals
Direct repeat
Tissue Distribution
RNA, Messenger
Promoter Regions, Genetic
3' Untranslated Regions
Gene
Repetitive Sequences, Nucleic Acid
030304 developmental biology
Genomic organization
0303 health sciences
Base Sequence
Three prime untranslated region
030302 biochemistry & molecular biology
Tumor Protein, Translationally-Controlled 1
Molecular biology
Phosphotransferases (Alcohol Group Acceptor)
genomic DNA
Gene Components
5' Untranslated Regions
Poly A
Sequence Alignment
Pseudogenes
Subjects
Details
- ISSN :
- 08887543 and 10898646
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....6363f04e5fb21acc3f0bb8c9df4bf198