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Evaluation of a Western Equine Encephalitis recombinant E1 protein for protective immunity and diagnostics
- Source :
- Antiviral Research. 64:85-92
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- The E1 and E2 glycoproteins of Western Equine Encephalitis (WEE) are candidate antigens for WEE subunit vaccine development. We have cloned the E1 gene of WEE virus and expressed it in Escherichia coli as inclusion bodies. The inclusion bodies were successfully solubilised, refolded and the immunogenicity of this unglycosylated protein was assessed in mice. Immunization of mice with recombinant E1 protein generated both humoral and cell-mediated immune responses, indicating the recombinant E1 protein is immunogenic. Challenge of E1-immunized mice with live WEE virus demonstrated little or no protection from this E. coli-derived non-glycosylated subunit.
- Subjects :
- Cellular immunity
Protein subunit
chemical and pharmacologic phenomena
Biology
Antibodies, Viral
Encephalitis Virus, Western Equine
Inclusion bodies
Virus
law.invention
Mice
Immune system
Viral Envelope Proteins
law
Virology
Escherichia coli
Animals
Inclusion Bodies
Pharmacology
Immunity, Cellular
Mice, Inbred BALB C
Immunogenicity
Antibodies, Monoclonal
Encephalomyelitis, Western Equine
Viral Vaccines
Recombinant Proteins
Humoral immunity
Recombinant DNA
Immunization
Subjects
Details
- ISSN :
- 01663542
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Antiviral Research
- Accession number :
- edsair.doi.dedup.....635da314450f2b956389a75b01e7f2f8