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Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes
- Source :
- The journal of cell biology (Online) 208 (2015): 581–596. doi:10.1083/jcb.201406040, info:cnr-pdr/source/autori:Viero G.; Lunelli L.; Passerini A.; Bianchini P.; Gilbert R.J.; Bernabo P.; Tebaldi T.; Diaspro A.; Pederzolli C.; Quattrone A./titolo:Three distinct ribosome assemblies modulated by translation are the building blocks of polysomes/doi:10.1083%2Fjcb.201406040/rivista:The journal of cell biology (Online)/anno:2015/pagina_da:581/pagina_a:596/intervallo_pagine:581–596/volume:208, The Journal of Cell Biology
- Publication Year :
- 2015
- Publisher :
- Rockefeller University Press, 2015.
-
Abstract
- Recurrent supra-ribosomal building blocks separated by ribosome-free mRNA regions form polysomes and reflect cellular translational activity.<br />Translation is increasingly recognized as a central control layer of gene expression in eukaryotic cells. The overall organization of mRNA and ribosomes within polysomes, as well as the possible role of this organization in translation are poorly understood. Here we show that polysomes are primarily formed by three distinct classes of ribosome assemblies. We observe that these assemblies can be connected by naked RNA regions of the transcript. We show that the relative proportions of the three classes of ribosome assemblies reflect, and probably dictate, the level of translational activity. These results reveal the existence of recurrent supra-ribosomal building blocks forming polysomes and suggest the presence of unexplored translational controls embedded in the polysome structure.
- Subjects :
- endocrine system
Messenger
FREE PROTEIN-SYNTHESIS
Biology
Ribosome
Article
Cell Line
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Polysome
Gene expression
Protein biosynthesis
Humans
RNA, Messenger
Ribosome profiling
Research Articles
ATOMIC-FORCE MICROSCOPY
030304 developmental biology
0303 health sciences
Messenger RNA
Tumor
RNA
Translation (biology)
Polyribosomes
Protein Biosynthesis
Cell Biology
DOUBLE-ROW POLYRIBOSOMES
Cell biology
MESSENGER-RNA TRANSLATION
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15408140 and 00219525
- Volume :
- 208
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....6353a4de22a2824dbcf6efcd0d52e79c