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Pituitary hormones dependent expression of insulin-like growth factors I and II in the immature hypophysectomized rat testis
- Source :
- Molecular endocrinology (Baltimore, Md.). 3(7)
- Publication Year :
- 1989
-
Abstract
- Since insulin-like growth factors I (IGF-I) and II (IGF-II) appeared involved in paracrine or autocrine regulation of both cell multiplication and differentiation of the rat testis, we have investigated the pituitary hormonal dependence of IGF-I and IGF-II mRNA production in the testis of immature hypophysectomized rats (22 days old) supplemented with highly purified FSH, LH, GH or PRL. Our data show that testicular expression of IGF-I mRNA as measured by dot-blot hybridization, is increased by LH, FSH or GH treatments of 7-, 6-, and 4-fold, respectively, above controls. Intensity of the signal was 3-fold lower after PRL treatment than in hypophysectomized control rats. On the contrary, IGF-II mRNA expression, was found low in the immature hypophysectomized rat testis and unmodified by any hormonal treatment. In contrast to the increase of IGF-I expression in the testis no significant change in the IGF-I plasma concentration was observed after LH or FSH supplementation. GH treatment, as expected, increased 4-fold the IGF-I plasma concentration of the experimental animals. Since we have previously shown that LH, FSH, and GH exhibit selective cell multiplication and differentiation in the testis of our animal model, it is proposed that testicular IGF-I expression could be the tissue response to pituitary hormone in these phenomena.
- Subjects :
- Male
endocrine system
Pituitary gland
medicine.medical_specialty
Hypophysectomy
medicine.medical_treatment
Biology
Testicle
Paracrine signalling
Endocrinology
Insulin-Like Growth Factor II
Somatomedins
Internal medicine
Testis
medicine
Animals
RNA, Messenger
Insulin-Like Growth Factor I
Autocrine signalling
Molecular Biology
Nucleic Acid Hybridization
Rats, Inbred Strains
General Medicine
Luteinizing Hormone
Blotting, Northern
Somatomedin
Prolactin
Rats
Pituitary Hormones
medicine.anatomical_structure
Insulin-like growth factor 2
Growth Hormone
biology.protein
RNA
Follicle Stimulating Hormone
Poly A
Hormone
Subjects
Details
- ISSN :
- 08888809
- Volume :
- 3
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Molecular endocrinology (Baltimore, Md.)
- Accession number :
- edsair.doi.dedup.....634939e885ab7d8e9b2ec289a35426ba