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Indirubin derivatives inhibit malignant lymphoid cell proliferation
- Source :
- Leukemialymphoma. 50(12)
- Publication Year :
- 2009
-
Abstract
- Indirubin-3'-monoxime (IO) is a derivative of indirubin, an active compound of a traditional Chinese medicinal recipe used to treat various inflammatory and malignant diseases. The main in vitro targets of IO (i.e. cyclin dependent kinases, glycogen synthase kinase-3beta, Stat 3 and Aryl hydrocarbon receptor) are regulators of lymphocyte activation. We investigated the interest of IO and its derivative 6-bromo-indirubin-3'oxime (6BIO) for inhibiting the growth of malignant lymphoid cells. IO (1-20 microM) induced cell cycle inhibition and cell death in malignant B- (IM9, Reh6) and T- (Jurkat, CEM-T) lymphoid cell lines depending to cell type, doses, and duration of treatment. IO and 6BIO (10 microM) treatment for 24 and 48 h were compared: 6BIO treatment resulted in a stronger cytotoxicity and more profound inhibition of cell proliferation. Taken together, these results showed that IO and, moreover, its derivative 6BIO may be potent antiproliferative agents in malignant lymphoid cells.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
Cancer Research
Indoles
Time Factors
Cell Survival
Blotting, Western
Apoptosis
Cell Cycle Proteins
Jurkat cells
Cell Line
chemistry.chemical_compound
Jurkat Cells
Cyclin-dependent kinase
Cell Line, Tumor
Cyclin E
Oximes
Autophagy
Humans
Lymphocytes
Cytotoxicity
Cell Proliferation
Antibiotics, Antineoplastic
biology
Dose-Response Relationship, Drug
Cell growth
Cell Cycle
Intracellular Signaling Peptides and Proteins
Hematology
Cyclin-Dependent Kinase 6
Cell cycle
Aryl hydrocarbon receptor
Flow Cytometry
Oncology
Biochemistry
chemistry
Cell culture
biology.protein
Cancer research
Indirubin
Cyclin-Dependent Kinase Inhibitor p27
Subjects
Details
- ISSN :
- 10292403
- Volume :
- 50
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Leukemialymphoma
- Accession number :
- edsair.doi.dedup.....63170789f63aa4c105c85f0980155e2d